Formation of Incision Complex in GG-NER

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R-HSA-5696395
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Homo sapiens
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After the XPC complex and the UV-DDB complex bind damaged DNA, a basal transcription factor TFIIH is recruited to the nucleotide excision repair (NER) site (Volker et al. 2001, Riedl et al. 2003). DNA helicases ERCC2 (XPD) and ERCC3 (XPB) are subunits of the TFIIH complex. ERCC2 unwinds the DNA around the damage in concert with the ATPase activity of ERCC3, creating an open bubble (Coin et al. 2007). Simultaneously, the presence of the damage is verified by XPA (Camenisch et al. 2006). The recruitment of XPA is partially regulated by PARP1 and/or PARP2 (King et al. 2012).

Two DNA endonucleases, ERCC5 (XPG) and the complex of ERCC1 and ERCC4 (XPF), are recruited to the open bubble structure to form the incision complex that will excise the damaged oligonucleotide from the affected DNA strand (Dunand-Sauthier et al. 2005, Zotter et al. 2006, Riedl et al. 2003, Tsodikov et al. 2007, Orelli et al. 2010). The RPA heterotrimer coats the undamaged DNA strand, thus protecting it from the endonucleolytic attack (De Laat et al. 1998).

Literature References
PubMed ID Title Journal Year
9716411 DNA-binding polarity of human replication protein A positions nucleases in nucleotide excision repair

de Laat, WL, Appeldoorn, E, Sugasawa, K, Weterings, E, Jaspers, NG, Hoeijmakers, JH

Genes Dev. 1998
23038248 Poly(ADP-ribose) contributes to an association between poly(ADP-ribose) polymerase-1 and xeroderma pigmentosum complementation group A in nucleotide excision repair

King, BS, Cooper, KL, Liu, KJ, Hudson, LG

J. Biol. Chem. 2012
19940136 The XPA-binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathways

Orelli, B, McClendon, TB, Tsodikov, OV, Ellenberger, T, Niedernhofer, LJ, Schärer, OD

J. Biol. Chem. 2010
14517266 The comings and goings of nucleotide excision repair factors on damaged DNA

Riedl, T, Hanaoka, F, Egly, JM

EMBO J. 2003
17466626 Distinct roles for the XPB/p52 and XPD/p44 subcomplexes of TFIIH in damaged DNA opening during nucleotide excision repair

Coin, F, Oksenych, V, Egly, JM

Mol. Cell 2007
17948053 Structural basis for the recruitment of ERCC1-XPF to nucleotide excision repair complexes by XPA

Tsodikov, OV, Ivanov, D, Orelli, B, Staresincic, L, Shoshani, I, Oberman, R, Schärer, OD, Wagner, G, Ellenberger, T

EMBO J. 2007
17000769 Recruitment of the nucleotide excision repair endonuclease XPG to sites of UV-induced dna damage depends on functional TFIIH

Zotter, A, Luijsterburg, MS, Warmerdam, DO, Ibrahim, S, Nigg, A, van Cappellen, WA, Hoeijmakers, JH, van Driel, R, Vermeulen, W, Houtsmuller, AB

Mol. Cell. Biol. 2006
16491090 Recognition of helical kinks by xeroderma pigmentosum group A protein triggers DNA excision repair

Camenisch, U, Dip, R, Schumacher, SB, Schuler, B, Naegeli, H

Nat. Struct. Mol. Biol. 2006
11511374 Sequential assembly of the nucleotide excision repair factors in vivo.

Volker, M, Moné, MJ, Karmakar, P, van Hoffen, A, Schul, W, Vermeulen, W, Hoeijmakers, JH, van Driel, R, van Zeeland, AA, Mullenders, LH

Mol Cell 2001
15590680 The spacer region of XPG mediates recruitment to nucleotide excision repair complexes and determines substrate specificity

Dunand-Sauthier, I, Hohl, M, Thorel, F, Jaquier-Gubler, P, Clarkson, SG, Schärer, OD

J. Biol. Chem. 2005
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