Deposition of new CENPA-containing nucleosomes at the centromere

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R-HSA-606279
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Homo sapiens
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Eukaryotic centromeres are marked by a unique form of histone H3, designated CENPA in humans. In human cells newly synthesized CENPA is deposited in nucleosomes at the centromere during late telophase/early G1 phase of the cell cycle. Once deposited, nucleosomes containing CENPA remain stably associated with the centromere and are partitioned equally to daughter centromeres during S phase. A current model proposes that pre-existing CENPA at the centromere drives recruitment of new CENPA, however this has not been proved.
The deposition process requires at least 3 complexes: the Mis18 complex, HJURP complex, and the RSF complex. HJURP binds newly synthesized CENPA-H4 tetramers before deposition and brings them to the centromere for deposition in new CENPA-containing nucleosomes. The exact mechanism of deposition remains unknown.

Literature References
PubMed ID Title Journal Year
19660450 Epigenetic specification of centromeres by CENP-A

Bernad, R, Sánchez, P, Losada, A

Exp Cell Res 2009
19410545 HJURP is a cell-cycle-dependent maintenance and deposition factor of CENP-A at centromeres

Dunleavy, EM, Roche, D, Tagami, H, Lacoste, N, Ray-Gallet, D, Nakamura, Y, Daigo, Y, Nakatani, Y, Almouzni-Pettinotti, G

Cell 2009
20080577 HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres

Shuaib, M, Ouararhni, K, Dimitrov, S, Hamiche, A

Proc Natl Acad Sci U S A 2010
17339380 Propagation of centromeric chromatin requires exit from mitosis

Jansen, LE, Black, BE, Foltz, DR, Cleveland, DW

J Cell Biol 2007
19410539 Frodos found: Behold the CENP-a "Ring" bearers

Mellone, BG, Zhang, W, Karpen, GH

Cell 2009
19410544 Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP

Foltz, DR, Jansen, LE, Bailey, AO, Yates, JR 3rd, Bassett, EA, Wood, S, Black, BE, Cleveland, DW

Cell 2009
19590885 At the right place at the right time: novel CENP-A binding proteins shed light on centromere assembly

Silva, MC, Jansen, LE

Chromosoma 2009
19629040 Focus on the centre: the role of chromatin on the regulation of centromere identity and function

Torras-Llort, M, Moreno-Moreno, O, Azorín, F

EMBO J 2009
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