NSUN2 methylates cytidine-34, cytidine-48 of unspliced tRNA(Leu)(CAA)

Stable Identifier
R-HSA-6782388
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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NSUN2 transfers a methyl group from S-adenosylmethionine to the 5 positions of cytidine-34 and cytidine-48 in tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012, Squires et al. 2012, Khoddami and Cairns 2013). Methylation of cytidine-34 occurs on the uspliced precursor tRNA(Leu)(CAA) (Brzezicha et al. 2006, Auxilien et al. 2012); methylation of cytidine-48 occurs on either the spliced or unspliced tRNA(Leu)(CAA) (Auxilien et al. 2012).

Literature References
PubMed ID Title Journal Year
23604283 Identification of direct targets and modified bases of RNA cytosine methyltransferases

Khoddami, V, Cairns, BR

Nat. Biotechnol. 2013
22995836 The human tRNA m (5) C methyltransferase Misu is multisite-specific

Auxilien, S, Guérineau, V, Szweykowska-Kulińska, Z, Golinelli-Pimpaneau, B

RNA Biol 2012
22344696 Widespread occurrence of 5-methylcytosine in human coding and non-coding RNA

Squires, JE, Patel, HR, Nousch, M, Sibbritt, T, Humphreys, DT, Parker, BJ, Suter, CM, Preiss, T

Nucleic Acids Res. 2012
17071714 Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA)

Brzezicha, B, Schmidt, M, Makalowska, I, Jarmolowski, A, Pienkowska, J, Szweykowska-Kulinska, Z

Nucleic Acids Res. 2006
Participants
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Catalyst Activity
Catalyst Activity
Title
tRNA (cytosine-5-)-methyltransferase activity of NSUN2 [nucleoplasm]
Physical Entity
Activity
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Authored
Reviewed
Created