Synthesis of wybutosine at G37 of tRNA(Phe)

Stable Identifier
R-HSA-6782861
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Pathway
Species
Homo sapiens
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Derivatives of wyosine are tricyclic bases found at nucleotide 37 of tRNA(Phe) in eukaryotes. The pathway of wybutosine synthesis begins with a templated guanosine residue and proceeds through 6 steps catalyzed by 5 enzymes: N1 methylation of guanosine, condensation of 1-methylguanosine with pyruvate to yield 4-demethylwyosine, addition of an aminocarboxypropyl group to yield yW-86, methylation of yW-86 to yield yW-72, methylation of yW-72 to yield yW-58, and methoxycarbonylation of yW-58 to yield wybutosine (reviewed in Young and Bandarian 2013, Perche-Letuvée et al. 2014). Wybutosine may further be modified by hydroxylation and methylation. Wyosine derivatives at position 37 of tRNAs participate in translational fidelity by stabilizing codon-anticodon pairing (Konevega et al. 2004) and preventing frameshifting (Waas et al. 2007).

Literature References
PubMed ID Title Journal Year
14681588 Purine bases at position 37 of tRNA stabilize codon-anticodon interaction in the ribosomal A site by stacking and Mg2+-dependent interactions

Konevega, AL, Soboleva, NG, Makhno, VI, Semenkov, YP, Wintermeyer, W, Rodnina, MV, Katunin, VI

RNA 2004
25629788 Wybutosine biosynthesis: structural and mechanistic overview

Perche-Letuvée, P, Molle, T, Forouhar, F, Mulliez, E, Atta, M

RNA Biol 2014
17623669 Role of a tRNA base modification and its precursors in frameshifting in eukaryotes

Waas, WF, Druzina, Z, Hanan, M, Schimmel, P

J. Biol. Chem. 2007
23856057 Radical mediated ring formation in the biosynthesis of the hypermodified tRNA base wybutosine

Young, AP, Bandarian, V

Curr Opin Chem Biol 2013
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