FANCD2:FANCI complex and UBE2T bind ICL-DNA associated with the FA core complex

Stable Identifier
R-HSA-6785342
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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The ID2 complex, composed of FANCD2 and FANCI, binds to branched DNA structures, such as stalled replication forks at DNA interstrand crosslinks (ICL-DNA) (Yuan et al. 2009, Longerich et al. 2009, Joo et al. 2011). The ID2 complex also interacts with the FA core complex component FANCL, activating the E3 ubiquitin ligase activity of FANCL (Rajendra et al. 2014, Longerich et al. 2014). Up to fifty FANCD2 molecules (ID2 complexes) may be recruited per one ICL, probably spreading to surrounding DNA (Douwel et al. 2014). The E2 ubiquitin ligase UBE2T is recruited to ICL-DNA by binding to the FANCL subunit of the FA core complex independently of the ID2 complex (Machida et al. 2006, Alpi et al. 2007, Hodson et al. 2014).

Literature References
PubMed ID Title Journal Year
17938197 UBE2T, the Fanconi anemia core complex, and FANCD2 are recruited independently to chromatin: a basis for the regulation of FANCD2 monoubiquitination

Alpi, A, Langevin, F, Mosedale, G, Machida, YJ, Dutta, A, Patel, KJ

Mol. Cell. Biol. 2007
19589784 FANCI binds branched DNA and is monoubiquitinated by UBE2T-FANCL

Longerich, S, San Filippo, J, Liu, D, Sung, P

J. Biol. Chem. 2009
24623813 Regulation of FANCD2 and FANCI monoubiquitination by their interaction and by DNA

Longerich, S, Kwon, Y, Tsai, MS, Hlaing, AS, Kupfer, GM, Sung, P

Nucleic Acids Res. 2014
24905007 The genetic and biochemical basis of FANCD2 monoubiquitination

Rajendra, E, Oestergaard, VH, Langevin, F, Wang, M, Dornan, GL, Patel, KJ, Passmore, LA

Mol. Cell 2014
24726325 XPF-ERCC1 acts in Unhooking DNA interstrand crosslinks in cooperation with FANCD2 and FANCP/SLX4

Klein Douwel, D, Boonen, RA, Long, DT, Szypowska, AA, Räschle, M, Walter, JC, Knipscheer, P

Mol. Cell 2014
24389026 Structure of the human FANCL RING-Ube2T complex reveals determinants of cognate E3-E2 selection

Hodson, C, Purkiss, A, Miles, JA, Walden, H

Structure 2014
16916645 UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation

Machida, YJ, Machida, Y, Chen, Y, Gurtan, AM, Kupfer, GM, D'Andrea, AD, Dutta, A

Mol Cell 2006
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