NOX2 generates superoxide anion from oxygen

Stable Identifier
R-HSA-6789092
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Phagocytic cells kill microorganisms by ingesting them into phagocytic vacuoles (phagosomes). Phagocytosis is accompanied by the activation of the NADPH oxidase (NOX2 complex), a multiprotein enzyme complex, that assembles in the phagosomal membrane (Winterbourn C et al. 2006). The NOX2 complex shuttles electrons from NADPH in the cytoplasm across the membrane to oxygen in the phagosomal lumen converting oxygen into the superoxide radical anion (O2.-). As this electron transfer creates a charge imbalance that would otherwise depolarize the membrane, NADPH oxidase activity is accompanied by activation of the V-ATPase and voltage-gated proton channel (Demaurex N & El Chemaly A 2010; El Chemaly A et al. 2010; Nunes P et al. 2013).

Defects in NADPH oxidase components are associated with chronic granulomatous disease (CGD) (de Oliveira-Junior EB et al. 2011). Phagocytic cells of CGD patients are unable to produce superoxide ion, and their efficiency in bacterial killing is significantly impaired (Johnston RB Jr et al. 1975; de Oliveira-Junior EB et al. 2011). In addition, macrophages from CGD patients exhibit abnormal function because these cells release higher levels of anti-inflammatory cytokines and lower levels of proinflammatory cytokines in response to bacterial stimuli (Rahman FZ et al. 2009).

Literature References
PubMed ID Title Journal Year
10029572 NADPH oxidase: an update

Babior, BM

Blood 1999
11970843 Assembly and activation of the neutrophil NADPH oxidase in granule membranes

Karlsson, A, Dahlgren, C

Antioxid. Redox Signal. 2002
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
superoxide-generating NADPH oxidase activity of NOX2 complex:RAC2:GTP [phagocytic vesicle membrane]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created