Box C/D snoRNP methylates ribonucleotides in pre-rRNA yielding 2'-O-methylribonucleotides

Stable Identifier
R-HSA-6790907
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The Fibrillarin (FBL) component of Box C/D small nucleolar ribonucleoprotein complexes (snoRNPs) transfers a methyl group from s-adenosylmethionine to the 2' hydroxyl group of ribonucleotides in precursor rRNAs (pre-rRNAs) in the nucleolus (Maden and Hughes 1997, inferred from mouse homologs in Cavaille and Bachellerie 1998, and inferred from homologues in Saccharomyces cerevisiae). The box C/D snoRNA component of the complex guides the methylation by base pairing with 10-21 nucleotide regions of the pre-rRNA (Filippova et al. 2015, inferred from mouse homologs in Cavaille and Bachellerie 1998). The protein components of the core box C/D snoRNP are FBL (homologous to NOP1 in yeast), NOP56, NOP58, and NHP2L1 (15.5K, homologous to SNU13 of yeast) (Lyman et al. 1999, Watkins et al. 2000, Watkins et al. 2002, Schultz et al. 2006, McKeegan et al. 2007). More than 100 nucleotide positions in human rRNAs are 2'-O-methylated and the human genome encodes more than 250 box C/D snoRNAs (Lestrade and Weber 2006), which are 50-300 nucleotides in length and contain box C (RUGAGA) and box D (CUGA) elements.

Literature References
PubMed ID Title Journal Year
17636026 A dynamic scaffold of pre-snoRNP factors facilitates human box C/D snoRNP assembly

McKeegan, KS, Debieux, CM, Boulon, S, Bertrand, E, Watkins, NJ

Mol. Cell. Biol. 2007
16782898 Protein-protein and protein-RNA contacts both contribute to the 15.5K-mediated assembly of the U4/U6 snRNP and the box C/D snoRNPs

Schultz, A, Nottrott, S, Watkins, NJ, Lührmann, R

Mol. Cell. Biol. 2006
16381836 snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs

Lestrade, L, Weber, MJ

Nucleic Acids Res. 2006
10606270 Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

Lyman, SK, Gerace, L, Baserga, SJ

RNA 1999
11081632 A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

Watkins, NJ, Ségault, V, Charpentier, B, Nottrott, S, Fabrizio, P, Bachi, A, Wilm, M, Rosbash, M, Branlant, C, Lührmann, R

Cell 2000
12417735 Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP

Watkins, NJ, Dickmanns, A, Lührmann, R

Mol. Cell. Biol. 2002
9512526 SnoRNA-guided ribose methylation of rRNA: structural features of the guide RNA duplex influencing the extent of the reaction

Cavaillé, J, Bachellerie, JP

Nucleic Acids Res. 1998
26085946 Modified Method of rRNA Structure Analysis Reveals Novel Characteristics of Box C/D RNA Analogues

Filippova, JA, Stepanov, GA, Semenov, DV, Koval, OA, Kuligina, EV, Rabinov, IV, Richter, VA

Acta Naturae 2015
9211966 Eukaryotic ribosomal RNA: the recent excitement in the nucleotide modification problem

Maden, BE, Hughes, JM

Chromosoma 1997
Participants
Participant Of
Event Information
Go Biological Process
Catalyst Activity
Catalyst Activity
Title
O-methyltransferase activity of Box C/D snoRNP [nucleoplasm]
Physical Entity
Activity
Inferred From
Authored
Reviewed
Created