Reactome | 21S pre-rRNA is nucleolytically processed at site E (site2a) to yield 18SE pre-rRNA

21S pre-rRNA is nucleolytically processed at site E (site2a) to yield 18SE pre-rRNA

Stable Identifier

R-HSA-6791222

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

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21S pre-rRNA is nucleolytically processed at site E (site2a) to yield 18SE pre-rRNA

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An unknown endonuclease in the nucleolus cleaves at site E (site 2a in mouse) of 21S pre-rRNA, yielding 18SE pre-rRNA (Preti et al. 2013, Sloan et al. 2013). Evidence also indicates that 18SE may also be produced by an exonucleolytic pathway (Carron et al. 2011, Sloan et al. 2013). BYSL (Bystin, ENG1), SKIV2L2 (MTR4), and the exonuclease activity of EXOSC10 (RRP6), all associated with the exosome, are required for formation of 18SE by the exonucleolytic pathway (Sloan et al. 2013). Sequencing indicates that 18SE molecules can have variable ends, presumably due to exonuclease activity (Preti et al. 2013).

Literature References

PubMed ID	Title	Journal	Year
23439679	Both endonucleolytic and exonucleolytic cleavage mediate ITS1 removal during human ribosomal RNA processing Sloan, KE, Mattijssen, S, Lebaron, S, Tollervey, D, Pruijn, GJ, Watkins, NJ	J. Cell Biol.	2013
20805244	Analysis of two human pre-ribosomal factors, bystin and hTsr1, highlights differences in evolution of ribosome biogenesis between yeast and mammals Carron, C, O'Donohue, MF, Choesmel, V, Faubladier, M, Gleizes, PE	Nucleic Acids Res.	2011
23482395	Gradual processing of the ITS1 from the nucleolus to the cytoplasm during synthesis of the human 18S rRNA Preti, M, O'Donohue, MF, Montel-Lehry, N, Bortolin-Cavaillé, ML, Choesmel, V, Gleizes, PE	Nucleic Acids Res.	2013