CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter

Stable Identifier
R-HSA-6810233
Type
Reaction [transition]
Species
Homo sapiens
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CDK7 phosphorylates serine-5 residues of heptad repeats (consensus YSPTSPS) in the C-terminal domain (CTD) of the large subunit (POLR2A) of RNA polymerase II. Serine-7 residues of the heptad repeats are also phosphorylated at promoters of snRNA genes (Egloff et al. 2007) and CDK7 is required for phosphorylation of serine-7 in vivo (Glover-Cutter et al. 2009). P-TEFb and DNA-PK are able to phosphorylate serine-7 in vitro (Glover-Cutter et al. 2009, Egloff et al. 2010). Impairment of CTD phosphorylation does not appear to affect transcription of snRNA genes but rather impairs 3' processing of the pre-snRNA (Medlin et al. 2003, Jacobs et al. 2004).

Literature References
PubMed ID Title Journal Year
12574128 The C-terminal domain of pol II and a DRB-sensitive kinase are required for 3' processing of U2 snRNA

Medlin, JE, Uguen, P, Taylor, A, Bentley, DL, Murphy, S

EMBO J. 2003
19667075 TFIIH-associated Cdk7 kinase functions in phosphorylation of C-terminal domain Ser7 residues, promoter-proximal pausing, and termination by RNA polymerase II

Glover-Cutter, K, Larochelle, S, Erickson, B, Zhang, C, Shokat, K, Fisher, RP, Bentley, DL

Mol. Cell. Biol. 2009
18079403 Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression

Egloff, S, O'Reilly, D, Chapman, RD, Taylor, A, Tanzhaus, K, Pitts, L, Eick, D, Murphy, S

Science 2007
14701755 Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing

Jacobs, EY, Ogiwara, I, Weiner, AM

Mol. Cell. Biol. 2004
20457598 The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain

Egloff, S, Szczepaniak, SA, Dienstbier, M, Taylor, A, Knight, S, Murphy, S

J. Biol. Chem. 2010
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Catalyst Activity
Title
protein serine/threonine kinase activity of RNA polymerase II (unphosphorylated):Initiation factors at promoter of snRNA gene [nucleoplasm]
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