Reactome | Pre-snRNA transcript initiation, Integrator binding, LEC binding

Pre-snRNA transcript initiation, Integrator binding, LEC binding

Stable Identifier

R-HSA-6814549

Type

Reaction [omitted]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

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Pre-snRNA transcript initiation, Integrator binding, LEC binding

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In an unknown order of events, RNA polymerase II initiates transcription and the Integrator complex (Baillat et al. 2005) and Little Elongation Complex (LEC, Hu et al. 2013) are recruited to phosphorylated RNA polymerase II (Egloff et al. 2010). The Integrator complex interacts with RPAP2, which binds phosphoserine-7 of the C-terminal domain (CTD) of RNA polymerase II and is required for recruitment of Integrator (Egloff et al. 2007, Egloff et al. 2012). RPAP2 interacts with the putative scaffold proteins RPRD1A and RPRD1B at the CTD (Ni et al. 2011, Ni et al. 2014) and DSIF is required for recruitment of Integrator (Skaar et al. 2015). The Integrator complex does not seem to play a significant role in subsequent elongation of the pre-snRNA transcript but is critical for processing of the 3' end of the pre-snRNA.

Literature References

PubMed ID	Title	Journal	Year
23932780	The little elongation complex functions at initiation and elongation phases of snRNA gene transcription Eissenberg, JC, Smith, ER, Lin, C, Hu, D, Shilatifard, A, Gao, X, Varberg, JM, Mohaghegh, N, Florens, LA, Saraf, A, Jackson, J, Washburn, MP, Garruss, AS	Mol. Cell	2013
18079403	Serine-7 of the RNA polymerase II CTD is specifically required for snRNA gene expression Tanzhaus, K, Chapman, RD, Murphy, S, Pitts, L, O'Reilly, D, Eick, D, Egloff, S, Taylor, A	Science	2007
22137580	Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes Laitem, C, Murphy, S, Kiss, T, Zaborowska, J, Egloff, S	Mol. Cell	2012
24997600	RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation Mosley, AL, Young, P, Li, J, Murphy, S, Olsen, JB, Zhong, G, He, H, Hunter, GO, Emili, A, Huang, H, Xu, C, Senisterra, GA, Min, J, Ni, Z, Kuo, WH, Guo, X, Greenblatt, JF, Loppnau, P, Arrowsmith, CH, Guo, H, Marcon, E, Tempel, W, Wan, C, El Bakkouri, M, Kuznetsova, OV, Sidhu, SS	Nat. Struct. Mol. Biol.	2014
20457598	The integrator complex recognizes a new double mark on the RNA polymerase II carboxyl-terminal domain Murphy, S, Dienstbier, M, Szczepaniak, SA, Egloff, S, Taylor, A, Knight, S	J. Biol. Chem.	2010
25675981	The Integrator complex controls the termination of transcription at diverse classes of gene targets Wu, X, Hughes, SH, Florens, LA, Saraf, A, Skaar, JR, Pagano, M, Ferris, AL, Khanna, KK, Washburn, MP	Cell Res.	2015
16239144	Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II Baillat, D, Cooch, N, Shilatifard, A, Shiekhattar, R, Näär, AM, Hakimi, MA	Cell	2005
22231121	Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B Guo, H, Marcon, E, Young, P, Li, J, Ruan, ED, Olsen, JB, Emili, A, Zhong, G, Ni, Z, Moffat, J, Guo, X, Greenblatt, JF	Transcription	2011