Integrator complex processes the 3' end of snRNA

Stable Identifier
R-HSA-6814555
Type
Reaction [omitted]
Species
Homo sapiens
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Transcription of the pre-snRNA extends through a conserved region, the 3' box, and terminates downstream. The heterodimeric subunits INTS9 and INTS11 within the Integrator complex form an endoribonuclease that cleaves the pre-snRNA at a location 5' to the 3' box (Baillat et al. 2005, Abrecht and Wagner 2012, Skaar et al. 2015), releasing the capped snRNA bound to the cap binding complex. Factors that bind the 5' cap of the pre-snRNA enhance processing at the 3' end (Hallais et al. 2013) and polyadenlyation factors PCF11 and SKU72 are required for transcription termination (O'Reilly et al. 2014). The remainder of the transcript downstream of the cleavage site is presumably degraded by exoribonuclease.

Literature References
PubMed ID Title Journal Year
24097444 Human snRNA genes use polyadenylation factors to promote efficient transcription termination

O'Reilly, D, Kuznetsova, OV, Laitem, C, Zaborowska, J, Dienstbier, M, Murphy, S

Nucleic Acids Res. 2014
22252320 snRNA 3' end formation requires heterodimeric association of integrator subunits

Albrecht, TR, Wagner, EJ

Mol. Cell. Biol. 2012
25675981 The Integrator complex controls the termination of transcription at diverse classes of gene targets

Skaar, JR, Ferris, AL, Wu, X, Saraf, A, Khanna, KK, Florens, LA, Washburn, MP, Hughes, SH, Pagano, M

Cell Res. 2015
16239144 Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II

Baillat, D, Hakimi, MA, Näär, AM, Shilatifard, A, Cooch, N, Shiekhattar, R

Cell 2005
24270878 CBC-ARS2 stimulates 3'-end maturation of multiple RNA families and favors cap-proximal processing

Hallais, M, Pontvianne, F, Andersen, PR, Clerici, M, Lener, D, Benbahouche, Nel H, Gostan, T, Vandermoere, F, Robert, MC, Cusack, S, Verheggen, C, Jensen, TH, Bertrand, E

Nat. Struct. Mol. Biol. 2013
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Catalyst Activity
Title
endoribonuclease activity of RNA polymerase II (phosphoserine-2,7):RPAP2:Integrator:LEC:CBCAP:capped pre-snRNA:Initiation factors:snRNA gene [nucleoplasm]
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