Reactome | Formation of Processive Complex

Formation of Processive Complex

Stable Identifier

R-HSA-69074

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

ReviewStatus

5/5

Locations in the PathwayBrowser

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Cell Cycle (Homo sapiens)

- Cell Cycle, Mitotic (Homo sapiens)
  - S Phase (Homo sapiens)
    - Synthesis of DNA (Homo sapiens)
      - DNA strand elongation (Homo sapiens)
        
        Lagging Strand Synthesis (Homo sapiens)
        
        Polymerase switching (Homo sapiens)
        
        Formation of Processive Complex (Homo sapiens)
        
        Leading Strand Synthesis (Homo sapiens)
        
        Polymerase switching (Homo sapiens)
        
        Formation of Processive Complex (Homo sapiens)

General

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The loading of proliferating cell nuclear antigen (PCNA) leads to recruitment of pol delta, the process of polymerase switching. Human PCNA is a homotrimer of 36 kDa subunits that form a toroidal structure. The loading of PCNA by RFC is a key event in the transition from the priming mode to the extension mode of DNA synthesis. The processive complex is composed of the pol delta holoenzyme and PCNA (Murakami et al.2010). Both PCNA and the DNA polymerase delta are needed for telomeric C-strand synthesis in a human telomere replication model (Lormand et al. 2013).

Literature References

PubMed ID	Title	Journal	Year
7378348	Purification of deoxyribonucleic acid polymerase delta from calf thymus: partial characterization of physical properties. So, AG, Downey, KM, Tan, CK, Lee, MY	Biochemistry	1980
7713898	A conserved region in the amino terminus of DNA polymerase delta is involved in proliferating cell nuclear antigen binding. Zhang, P, Toomey, NL, Zhang, SJ, Zeng, XR, Lee, MY, Chuang, RY, Chang, LS	J Biol Chem	1995
8104944	Interaction of proliferating cell nuclear antigen with yeast DNA polymerase delta. Campbell, JL, Brown, WC	J Biol Chem	1993
1682322	Saccharomyces cerevisiae replication factor C. II. Formation and activity of complexes with the proliferating cell nuclear antigen and with DNA polymerases delta and epsilon. Burgers, PM	J Biol Chem	1991
1974050	Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1. Hurwitz, J, Lee, SH	Proc Natl Acad Sci U S A	1990
10751307	Identification of a fourth subunit of mammalian DNA polymerase delta. Rodriguez-Belmonte, EM, Liu, L, Lee, MY, Mo, J	J Biol Chem	2000
9191022	DNA polymerase delta, an essential enzyme for DNA transactions. Hubscher, U, Hindges, R	Biol Chem	1997
1671046	Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase alpha and delta during initiation of leading and lagging strand synthesis. Stillman, B, Tsurimoto, T	J Biol Chem	1991