Phosphorylation and activation of CHEK2 by ATM

Stable Identifier
R-HSA-69891
Type
Reaction
Species
Homo sapiens
Compartment
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Summation

Activated ATM phosphorylates CHEK2 (CHK2, Cds1) on threonine residue T68 (Matsuoka et al. 2000, Melchionna et al. 2000). The presence of BRCA1 and TP53BP1 positively regulates ATM-mediated phosphorylation of CHEK2 (Wang et al. 2002, Foray et al. 2003). ATM-mediated phosphorylation causes formation of CHEK2 dimers and dissociation of CHEK2 from chromatin (Li and Stern 2005).

Literature References
PubMed ID Title Journal Year
16150728 DNA damage regulates Chk2 association with chromatin

Li, J, Stern, DF

J. Biol. Chem. 2005
11025670 Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1

Melchionna, R, Chen, XB, Blasina, A, McGowan, CH

Nat. Cell Biol. 2000
12364621 53BP1, a mediator of the DNA damage checkpoint.

Wang, B, Matsuoka, S, Carpenter, PB, Elledge, SJ

Science 2002
10973490 Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro

Matsuoka, S, Rotman, G, Ogawa, A, Shiloh, Y, Tamai, K, Elledge, SJ

Proc Natl Acad Sci U S A 2000
12773400 A subset of ATM- and ATR-dependent phosphorylation events requires the BRCA1 protein.

Foray, N, Marot, D, Gabriel, A, Randrianarison, V, Perricaudet, M, Ashworth, A, Jeggo, P

EMBO J 2003
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein serine/threonine kinase activity of DNA DNA DSBs:p-MRN:p-S1981,Ac-K3016-ATM:KAT5:K63PolyUb-K14,K16,p-S139-H2AFX,Me2K21-HIST1H4A-Nucleosome:p-5T-MDC1:p-S102-WHSC1:RNF8:Zn2+:SUMO1:p-T4827-HERC2:UBE2N:UBE2V2:RNF168:PIAS4:p-S25,S1778-TP53BP1:p-4S,2T-BRCA1-A complex:CHEK2 [nucleoplasm]
Physical Entity
Activity
Orthologous Events
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Reviewed