1,3-bisphospho-D-glycerate + NADH + H+ <=> D-glyceraldehyde 3-phosphate + Orthophosphate + NAD+

Stable Identifier
R-HSA-70482
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The reversible reduction of 1,3-bisphosphoglycerate to form glyceraldehyde-3-phosphate is catalyzed by cytosolic glyceraldehyde-3-phosphate dehydrogenase tetramer.

There are multiple human glyceraldehyde 3-phosphate dehydrogenase-like pseudogenes, but only one glyceraldehyde 3-phosphate dehydrogenase gene expressed in somatic tissue (Benham and Povey 1989). Consistent with this conclusion, the homogeneous enzymes purified from various human tissues had indistinguishable physical and immunochemical properties (Heinz and Freimuller 1982), and studies of human erythrocytes of various ages suggested that variant forms of the enzyme arise as a result of post-translational modifications (Edwards et al. 1976). There is, however, an authentic second isoform of glyceraldehyde 3-phosphate dehydrogenase whose expression is confined to spermatogenic cells of the testis (Welch et al. 2000).

Literature References
PubMed ID Title Journal Year
183598 Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals.

Edwards, YH, Clark, P, Harris, H

Ann Hum Genet 1976
10714828 Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells.

Welch, JE, Brown, PL, O'Brien, DA, Magyar, PL, Bunch, DO, Mori, C, Eddy, EM

J Androl 2000
3170585 Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene

Ercolani, L, Florence, B, Denaro, M, Alexander, M

J Biol Chem 1988
7144574 Glyceraldehyde-3-phosphate dehydrogenase from human tissues.

Heinz, F, Freimüller, B

Methods Enzymol 1983
Participants
Participant Of
hasEvent
Event Information
Catalyst Activity
Catalyst Activity
Title
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity of GAPDH tetramers [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
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