Phosphoenolpyruvate + H2O <=> 2-Phospho-D-glycerate

Stable Identifier
R-HSA-70494
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Cytosolic enolase dimer catalyzes the reversible reaction of phosphoenolpyruvate and water to form 2-phosphoglycerate. Three enolase isozymes have been purified and biochemically characterized. The alpha isoform is widely expressed (Giallongo et al. 1986). The beta isoform is expressed in muscle. Evidence for its function in vivo in humans comes from studies of a patient in whom a point mutation in the gene encoding the enzyme was associated specifically with reduced enolase activity in muscle extracts, and with other symptoms consistent with a defect in glycolysis (Comi et al. 2001). The gamma isoform of human enolase is normally expressed in neural tissue. It is not known to have distinctive biochemical functions, but is of possible clinical interest as a marker of some types of neuroendocrine and lung tumors (McAleese et al. 1988). Verma and Kurl (1993) identifed a possible fourth isoform, a "lung-specific" enolase whose expression is increased in response to dexamethasone treatment. The protein has not been biochemically characterized, however, nor have the levels of mRNA and protein in other tissues been examined. Thus, the observation that this protein is particularly similar in its predicted amino acid sequence to a duck crystallin (Wistow et al. 1988) raises the possibility that its normal function is unrelated to glycolysis.

Literature References
PubMed ID Title Journal Year
3529090 Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase

Giallongo, A, Feo, S, Moore, R, Croce, CM, Showe, LC

Proc Natl Acad Sci U S A 1986
3208766 Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE)

McAleese, SM, Dunbar, B, Fothergill, JE, Hinks, LJ, Day, IN

Eur J Biochem 1988
11506403 Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis

Comi, GP, Fortunato, F, Lucchiari, S, Bordoni, A, Prelle, A, Jann, S, Keller, A, Ciscato, P, Galbiati, S, Chiveri, L, Torrente, Y, Scarlato, G, Bresolin, N

Ann Neurol 2001
Participants
Participant Of
hasEvent
Event Information
Catalyst Activity
Catalyst Activity
Title
phosphopyruvate hydratase activity of enolase dimer [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Reviewed