LDH tetramer oxidises LACT to PYR

Stable Identifier
R-HSA-70510
Type
Reaction
Species
Homo sapiens
Compartment
Synonyms
(S)-Lactate + NAD+ <=> Pyruvate + NADH + H+
Locations in the PathwayBrowser
Summation

Cytosolic lactate dehydrogenase catalyzes the freely reversible reaction of lactate and NAD+ to form pyruvate and NADH + H+. In liver parenchymal cells, this reaction allows lactate from red blood cells and exercising muscle to be converted to pyruvate which in turn is typically used for gluconeogenesis which also consumes the NADH from the reaction.

Lactate dehydrogenase is active as a tetramer. Two isoforms of lactate dehydrogenase, A and B, are widely expressed in human tissues, and all five tetramers - A4, A3B, A2B2, AB3, and B4 - are found (Read et al. 2001; Sakai et al. 1987; Yu et al. 2001). A third isoform, C, and its tetramer, C4, are found in testis (Millan et al. 1987; LeVan & Goldberg 1991). A fourth isoform, LDHAL6A, is less fully characterized than these others but limited data suggest that it may be testis-specific (Chen et al. 2009).

Literature References
PubMed ID Title Journal Year
18351441 Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA)

Chen, X, Gu, X, Shan, Y, Tang, W, Yuan, J, Zhong, Z, Wang, Y, Huang, W, Wan, B, Yu, L

Mol. Biol. Rep. 2009
3435492 The cDNA and protein sequences of human lactate dehydrogenase B

Sakai, I, Sharief, FS, Pan, YC, Li, SS

Biochem J 1987
2440048 Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence

Millan, JL, Driscoll, CE, LeVan, KM, Goldberg, E

Proc. Natl. Acad. Sci. U.S.A. 1987
11377399 Selective active site inhibitors of human lactate dehydrogenases A4, B4, and C4

Yu, Y, Deck, JA, Hunsaker, LA, Deck, LM, Royer, RE, Goldberg, E, Vander Jagt, DL

Biochem Pharmacol 2001
1996957 Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli

LeVan, KM, Goldberg, E

Biochem. J. 1991
11276087 Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase

Read, JA, Winter, VJ, Eszes, CM, Sessions, RB, Brady, RL

Proteins 2001
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
L-lactate dehydrogenase activity of LDH tetramer [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Reviewed