PXLP-K314-GPT transaminates L-Ala to form PYR

Stable Identifier
R-HSA-70523
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
alanine + alpha-ketoglutarate <=> pyruvate + glutamate [GPT]
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Cytosolic glutamic-pyruvate transaminase (alanine aminotransferase) (GPT) catalyzes the reversible reaction of alanine and 2-oxoglutarate (alpha-ketoglutarate) to form pyruvate and glutamate (Sohocki et al. 1997; Yang et al. 2002). The active form of the enzyme is a dimer (Ishiguro et al. 1991) and is inferred to have a molecule of pyridoxal phosphate associated with each monomer. This reaction allows the synthesis of alanine from intermediates of glucose metabolism in a well-fed person. Under fasting conditions, alanine, derived from protein breakdown, can be converted to pyruvate and used to synthesize glucose via the gluconeogenic pathway in liver, or fully oxidized via the TCA cycle in other tissues.

Literature References
PubMed ID Title Journal Year
1931970 Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods

Ishiguro, M, Takio, K, Suzuki, M, Oyama, R, Matsuzawa, T, Titani, K

Biochemistry 1991
9119391 Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites.

Sohocki, MM, Sullivan, LS, Harrison, WR, Sodergren, EJ, Elder, FF, Weinstock, G, Tanase, S, Daiger, SP

Genomics 1997
11863375 cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase

Yang, RZ, Blaileanu, G, Hansen, BC, Shuldiner, AR, Gong, DW

Genomics 2002
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
L-alanine:2-oxoglutarate aminotransferase activity of PXLP-K314-GPT dimer [cytosol]
Physical Entity
Activity
Orthologous Events