glutamate + NAD(P)+ => alpha-ketoglutarate + NH4+ + NAD(P)H + H+ [GLUD1]

Stable Identifier
R-HSA-70600
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Mitochondrial glutamate dehydrogenase 1 (GLUD1) catalyzes the reversible reaction of glutamate and NAD(P)+ to form 2-oxoglutarate, NAD(P)H + H+, and ammonia (Fang et al. 2002). Mature GLUD1 protein lacks the 53 aminoterminal residues of the nascent protein (Julliard and Smith 1979), which function as a mitochondrial import signal. The active form of the enzyme is a hexamer, allosterically activated by ADP and inhibited by GTP (Fang et al. 2002; Smith et al. 2002).

Literature References
PubMed ID Title Journal Year
11903050 Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations

Fang, J, Hsu, BY, MacMullen, CM, Poncz, M, Smith, TJ, Stanley, CA

Biochem J 2002
429360 Partial amino acid sequence of the glutamate dehydrogenase of human liver and a revision of the sequence of the bovine enzyme

Julliard, JH, Smith, EL

J Biol Chem 1979
12054821 The structure of apo human glutamate dehydrogenase details subunit communication and allostery

Smith, TJ, Schmidt, T, Fang, J, Wu, J, Siuzdak, G, Stanley, CA

J Mol Biol 2002
Participants
Participant Of
Event Information
Catalyst Activity
Catalyst Activity
Title
glutamate dehydrogenase [NAD(P)+] activity of GLUD hexamer [mitochondrial matrix]
Physical Entity
Activity
This event is regulated
Orthologous Events
Cross References
Rhea