glutamine + H2O => glutamate + NH4+ [GLS]

Stable Identifier
R-HSA-70609
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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Mitochondrial glutaminase (GLS) catalyzes the hydrolysis of glutamine to yield glutamate and ammonia. Two GLS enzymes have been identified, one abundantly expressed in the liver (GLS - Elgadi et al. 1999) and one abundantly expressed in kidney (GLS2 - Gomez-Fabre et al. 2000). Their biochemical properties are similar. The enzymes are inferred to function as dimers based on unpublished crystallographic data for GLS (PDB 3CZD) and studies of glutaminase enzyme purified from Ehrlich Ascites cells (Quesada et al. 1988).

Literature References
PubMed ID Title Journal Year
3214421 Purification of phosphate-dependent glutaminase from isolated mitochondria of Ehrlich ascites-tumour cells

Quesada, AR, Sánchez-Jiménez, F, Perez-Rodriguez, J, Marquez, J, Medina, MA, Nunez De Castro, I

Biochem J 1988
10620514 Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase

Gomez-Fabre, PM, Aledo, JC, Del Castillo-Olivares, A, Alonso, FJ, Nunez De Castro, I, Campos, JA, Marquez, J

Biochem J 2000
11015561 Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing

Elgadi, KM, Meguid, RA, Qian, M, Souba, WW, Abcouwer, SF

Physiol Genomics 1999
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
glutaminase activity of GLS dimers [mitochondrial matrix]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored
Reviewed