Formation of the Spliceosomal B Complex

Stable Identifier
Reaction [binding]
Homo sapiens
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The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References
PubMed ID Title Journal Year
12477934 Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.

Hartmuth, K, Urlaub, H, Lührmann, R, Gentzel, M, Wilm, M, Vornlocher, HP, Will, CL

Proc Natl Acad Sci U S A 2002
12176931 Large-scale proteomic analysis of the human spliceosome.

Lamond, AI, Mann, M, Rappsilber, J, Ryder, U

Genome Res 2002
16809785 Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

Will, CL, Lührmann, R, Deckert, J, Boehringer, D, Urlaub, H, Stark, H, Behzadnia, N, Kastner, B, Hartmuth, K

Mol. Cell. Biol. 2006
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