Reactome | Formation of the Spliceosomal B Complex

Formation of the Spliceosomal B Complex

Stable Identifier

R-HSA-72127

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

Locations in the PathwayBrowser

Expand all

General

SBML | | PDF

SVG | | PPTX | SBGN

Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References

PubMed ID	Title	Journal	Year
12477934	Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. Hartmuth, K, Urlaub, H, Lührmann, R, Gentzel, M, Wilm, M, Vornlocher, HP, Will, CL	Proc Natl Acad Sci U S A	2002
12176931	Large-scale proteomic analysis of the human spliceosome. Lamond, AI, Mann, M, Rappsilber, J, Ryder, U	Genome Res	2002
16809785	Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions Will, CL, Lührmann, R, Deckert, J, Boehringer, D, Urlaub, H, Stark, H, Behzadnia, N, Kastner, B, Hartmuth, K	Mol. Cell. Biol.	2006