Formation of the Spliceosomal B Complex

Stable Identifier
R-HSA-72127
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References
PubMed ID Title Journal Year
12176931 Large-scale proteomic analysis of the human spliceosome.

Rappsilber, J, Ryder, U, Lamond, AI, Mann, M

Genome Res 2002
12477934 Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.

Hartmuth, K, Urlaub, H, Vornlocher, HP, Will, CL, Gentzel, M, Wilm, M, Lührmann, R

Proc Natl Acad Sci U S A 2002
16809785 Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

Deckert, J, Hartmuth, K, Boehringer, D, Behzadnia, N, Will, CL, Kastner, B, Stark, H, Urlaub, H, Lührmann, R

Mol. Cell. Biol. 2006
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