Formation of the Spliceosomal B Complex

Stable Identifier
R-HSA-72127
Type
Reaction [binding]
Species
Homo sapiens
Compartment
nucleoplasm
Locations in the PathwayBrowser
Expand all
General
SVG | 
PNG
Low
Medium
High
 | PPTX  | SBGN
Formation of the Spliceosomal B Complex
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References
PubMed ID Title Journal Year
12176931 Large-scale proteomic analysis of the human spliceosome.

Rappsilber, J, Ryder, U, Lamond, AI, Mann, M

Genome Res 2002
12477934 Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.

Hartmuth, K, Urlaub, H, Vornlocher, HP, Will, CL, Gentzel, M, Wilm, M, Lührmann, R

Proc Natl Acad Sci U S A 2002
16809785 Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

Deckert, J, Hartmuth, K, Boehringer, D, Behzadnia, N, Will, CL, Kastner, B, Stark, H, Urlaub, H, Lührmann, R

Mol. Cell. Biol. 2006
Participants
Input
Output
Participant Of
hasEvent
Orthologous Events
Formation of the Spliceosomal B Complex (Bos taurus)
Formation of the Spliceosomal B Complex (Canis familiaris)
Formation of the Spliceosomal B Complex (Mus musculus)
Formation of the Spliceosomal B Complex (Rattus norvegicus)
Formation of the Spliceosomal B Complex (Sus scrofa)
Formation of the Spliceosomal B Complex (Xenopus tropicalis)
Authored
Krainer, AR  (2003-06-05)
Created
Krainer, AR  (2003-06-05)