Reactome | Formation of the Spliceosomal B Complex

Formation of the Spliceosomal B Complex

Stable Identifier

R-HSA-72127

Type

Reaction

Species

Homo sapiens

Compartment

nucleoplasm

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Summation

The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References

PubMed ID	Title	Journal	Year
12176931	Large-scale proteomic analysis of the human spliceosome.	Genome Res	2002
12477934	Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method.	Proc Natl Acad Sci U S A	2002
16809785	Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions	Mol. Cell. Biol.	2006

Participants

Input

Output

Participant Of

hasEvent

mRNA Splicing - Major Pathway

Orthologous Events

Formation of the Spliceosomal B Complex (Bos taurus)

Formation of the Spliceosomal B Complex (Canis familiaris)

Formation of the Spliceosomal B Complex (Mus musculus)

Formation of the Spliceosomal B Complex (Rattus norvegicus)

Formation of the Spliceosomal B Complex (Sus scrofa)