Reactome | Formation of the Spliceosomal B Complex

Formation of the Spliceosomal B Complex

Stable Identifier

R-HSA-72127

Type

Reaction [binding]

Species

Homo sapiens

Compartment

nucleoplasm

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The formation of the B complex is ATP-dependent, and both the 5' and 3' splice sites are essential for B complex assembly. The U4 and U6 snRNPS are extensively base-paired, and this U4:U6 complex associates with the U5 snRNP to form a tri-snRNP particle. This tri-snRNP particle then binds to the spliceosomal A complex, to form the spliceosomal B complex.

Literature References

PubMed ID	Title	Journal	Year
12176931	Large-scale proteomic analysis of the human spliceosome. Rappsilber, J, Ryder, U, Lamond, AI, Mann, M	Genome Res	2002
12477934	Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method. Hartmuth, K, Urlaub, H, Vornlocher, HP, Will, CL, Gentzel, M, Wilm, M, Lührmann, R	Proc Natl Acad Sci U S A	2002
16809785	Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions Deckert, J, Hartmuth, K, Boehringer, D, Behzadnia, N, Will, CL, Kastner, B, Stark, H, Urlaub, H, Lührmann, R	Mol. Cell. Biol.	2006