Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S

Stable Identifier
Homo sapiens
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The cap-binding complex is constituted by the initiation factors eIF4A, eIF4G and eIF4E. First, eIF4E must be released from the inactive eIF4E:4E-BP complex. Then eIF4A interacts with eIF4G, and eIF4E binds to the amino-terminal domain of eIF4G, resulting in the formation of the cap-binding complex eIF4F. eIF4A together with eIF4B or eIF4H is thought to unwind RNA secondary structures near the 5'-end of the mRNA. The translation initiation complex is formed when the 43S complex binds the cap-bound mRNA.

Literature References
PubMed ID Title Journal Year
592399 Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors.

Trachsel, H, Erni, B, Schreier, MH, Staehelin, T

J Mol Biol 1978
6853548 New initiation factor activity required for globin mRNA translation.

Grifo, JA, Tahara, SM, Morgan, MA, Shatkin, AJ, Merrick, WC

J Biol Chem 1983
641056 The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.

Benne, R, Hershey, JW

J Biol Chem 1978
9732867 Eukaryotic ribosomes require initiation factors 1 and 1A to locate initiation codons.

Pestova, TV, Borukhov, SI, Hellen, CU

Nature 1999
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Orthologous Events