FGAR + L-Glutamine + ATP + H2O => FGAM + L-Glutamate + ADP + Pi

Stable Identifier
R-HSA-73812
Type
Reaction
Species
Homo sapiens
Compartment
Synonyms
5'-phosphoribosylformylglycinamide (FGAR) + L-glutamine + ATP + H2O => 5'-phosphoribosylformylglycinamidine (FGAM) + L-glutamate + adenosine 5'-diphosphate + orthophosphate
Locations in the PathwayBrowser
Summation

The irreversible transfer of an amino group from L-glutamine to 5'-phosphoribosylformylglycinamide (FGAR), forming 5'-phosphoribosylformylglycinamidine (FGAM) and glutamate, accompanied by the conversion of ATP to ADP and orthophosphate, is catalyzed by phosphoribosylformylglycinamidine synthetase. The human enzyme has been purified and characterized biochemically (Barnes et al. 1994). Fluoresence microscopy studies of cultured human cells have shown that the enzyme is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (An et al. 2008).

Literature References
PubMed ID Title Journal Year
8110788 Purification of, generation of monoclonal antibodies to, and mapping of phosphoribosyl N-formylglycinamide amidotransferase

Barnes, TS, Bleskan, JH, Hart, IM, Walton, KA, Barton, JW, Patterson, D

Biochemistry 1994
18388293 Reversible compartmentalization of de novo purine biosynthetic complexes in living cells

An, S, Kumar, R, Sheets, ED, Benkovic, SJ

Science 2008
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
phosphoribosylformylglycinamidine synthase activity of PFAS [cytosol]
Physical Entity
Activity
Orthologous Events
Cross References
Rhea
Authored