Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA

Stable Identifier
R-HSA-77083
Type
Reaction [transition]
Species
Homo sapiens
Compartment
nucleoplasm
ReviewStatus
5/5
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Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA
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The diphosphate 5'-end of the mRNA is joined to the GMP, releasing it from the enzyme. At this time, it is unclear how the RNA diphosphate end is transferred from the active site of the triphosphatase to the guanylyltransferase site. The covalent enzyme-GMP complex can form in the absence of RNA.
Guanylyltransferase (GT) catalyzed second reaction can be represented as:ppN(pN)n + GTP -> GpppN(pN)n + PPi

(Yamada-Okabe et al. 1998).

Literature References
PubMed ID Title Journal Year
9512541 Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme.

Yamada-Okabe, H, Shimmi, O, Arisawa, M, Yamada-Okabe, T, Doi, R

Nucleic Acids Res 1998
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Catalyst Activity

mRNA guanylyltransferase activity of Capping complex (intermediate) [nucleoplasm]

Physical Entity
Capping complex (intermediate) [nucleoplasm] (Homo sapiens)
Activity
mRNA guanylyltransferase activity (GO:0004484)
Orthologous Events
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Bos taurus)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Caenorhabditis elegans)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Canis familiaris)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Danio rerio)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Dictyostelium discoideum)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Drosophila melanogaster)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Gallus gallus)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Mus musculus)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Plasmodium falciparum)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Rattus norvegicus)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Saccharomyces cerevisiae)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Schizosaccharomyces pombe)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Sus scrofa)
Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA (Xenopus tropicalis)
Authored
Buratowski, S  (2003-10-15)
Created
Buratowski, S  (2003-10-15)
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