Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA

Stable Identifier
R-HSA-77083
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The diphosphate 5'-end of the mRNA is joined to the GMP, releasing it from the enzyme. At this time, it is unclear how the RNA diphosphate end is transferred from the active site of the triphosphatase to the guanylyltransferase site. The covalent enzyme-GMP complex can form in the absence of RNA.
Guanylyltransferase (GT) catalyzed second reaction can be represented as:ppN(pN)n + GTP -> GpppN(pN)n + PPi

(Yamada-Okabe et al. 1998).

Literature References
PubMed ID Title Journal Year
9512541 Isolation and characterization of a human cDNA for mRNA 5'-capping enzyme.

Yamada-Okabe, T, Doi, R, Shimmi, O, Arisawa, M, Yamada-Okabe, H

Nucleic Acids Res 1998
Participants
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
mRNA guanylyltransferase activity of Capping complex (intermediate) [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Created
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