Phosphorylation of JAK2

Stable Identifier
R-HSA-873919
Type
Reaction
Species
Homo sapiens
Compartment
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IFN-gamma binding to the receptor complex, induces JAK2 autophosphorylation and activation. Like all protein tyrosine kinases (PTKs) JAK2 activity also depends on the phosphorylation of tandem tyrosine residues within the activation loop that results in the removal of the activation loop from the active site. Multiple phosphorylation sites have been identified in JAK2 (tyrosines 221, 570, 868, 966, 972, 1007 and 1008 ) of which phosphorylation of tyrosine 1007 is essential for kinase activity. Tyrosine 1007 is in the activation loop and phosphorylation allows access of the catalytic loop to the ATP in the ATP binding domain. Of all the predicted phoshorylation sites only tyrosine 1007 is represented in the reaction.

Literature References
PubMed ID Title Journal Year
15530433 Determination of the transphosphorylation sites of Jak2 kinase

Matsuda, T, Feng, J, Witthuhn, BA, Sekine, Y, Ihle, JN

Biochem Biophys Res Commun 2004
9111318 Activation of Jak2 catalytic activity requires phosphorylation of Y1007 in the kinase activation loop

Feng, J, Witthuhn, BA, Matsuda, T, Kohlhuber, F, Kerr, IM, Ihle, JN

Mol Cell Biol 1997
15143187 Autophosphorylation of JAK2 on tyrosines 221 and 570 regulates its activity

Argetsinger, LS, Kouadio, JL, Steen, H, Stensballe, A, Jensen, ON, Carter-Su, C

Mol Cell Biol 2004
20304997 Tyrosines 868, 966, and 972 in the kinase domain of JAK2 are autophosphorylated and required for maximal JAK2 kinase activity

Argetsinger, LS, Stuckey, JA, Robertson, SA, Koleva, RI, Cline, JM, Marto, JA, Myers MG, Jr, Carter-Su, C

Mol Endocrinol 2010
Participants
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Catalyst Activity
Catalyst Activity
Title
protein tyrosine kinase activity of IFNG:IFNGR1:JAK1:IFNGR2:JAK2 [plasma membrane]
Physical Entity
Activity
Orthologous Events
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