2-oxoglutarate + NADPH + H+ => (R)-2-hydroxyglutarate + NADP+ [mutant IDH1]

Stable Identifier
R-HSA-880053
Type
Reaction [transition]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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2-oxoglutarate + NADPH + H+ => (R)-2-hydroxyglutarate + NADP+ [mutant IDH1]
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Mutant forms of IDH1 in which the arginine residue at position 132 has been replaced by histidine, cystine, leucine, or serine catalyze the reaction of 2-oxoglutarate and NADPH + H+ to form (R)-2-hydroxyglutarate and NADP+. Like normal IDH1, the mutant enzyme forms a dimer located in the cytosol (Dang et al. 2009).

Such mutations occur frequently as a somatic event in human glioblastomas (Parsons et al. 2008). Cells expressing the mutant protein accumulate elevated levels of 2-hydroxyglutarate, probably in the cytosol as IDH1 is a cytosolic enzyme. The fate of the 2-hydroxyglutarate is unclear, but the high frequency with which the mutation is found in surveys of primary tumors is consistent with the possibility that it is advantageous to the tumor cells (Dang et al. 2009).

Literature References
PubMed ID Title Journal Year
19935646 Cancer-associated IDH1 mutations produce 2-hydroxyglutarate

Keenan, MC, White, DW, Su, SM, Liau, LM, Yen, KE, Bittinger, MA, Gross, S, Fantin, VR, Dang, L, Thompson, CB, Jin, S, Cantley, LC, Rabinowitz, JD, Ward, PS, Bennett, BD, Jang, HG, Marks, KM, Prins, RM, Vander Heiden, MG, Driggers, EM

Nature 2009
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Catalyst Activity

(R)-2-hydroxyglutarate dehydrogenase activity of IDH1 R132mutant dimers [cytosol]

Physical Entity
IDH1 R132mutant dimers [cytosol] (Homo sapiens)
Activity
(R)-2-hydroxyglutarate dehydrogenase activity (GO:0051990)
Disease
Name Identifier Synonyms
glioblastoma multiforme DOID:3068 GBM, adult glioblastoma multiforme, grade IV adult Astrocytic tumor, primary glioblastoma multiforme, spongioblastoma multiforme
Cross References
Rhea
Authored
D'Eustachio, P  (2010-06-26)
Reviewed
Jassal, B  (2010-11-09)
Rush, MG  (2011-01-31)
Created
D'Eustachio, P  (2010-06-26)
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