UBA1 conjugates ubiquitin to cytosolic E2 enzymes

Stable Identifier
R-HSA-8852129
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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In the cytosol, the UBA1-ubiquitin thiol ester conjugate transfers ubiquitin from UBA1 to an internal cysteine residue of the E2 enzyme, forming a thiol ester conjugate between ubiquitin and the cysteine residue of E2 (Jin et al. 2007, inferred from the rabbit homologue in Hershko et al. 1983). The E2 then disengages from UBA1 (Eletr et al. 2005).

Literature References
PubMed ID Title Journal Year
6305978 Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown

Hershko, A, Heller, H, Elias, S, Ciechanover, A

J. Biol. Chem. 1983
17597759 Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging

Jin, J, Li, X, Gygi, SP, Harper, JW

Nature 2007
16142244 E2 conjugating enzymes must disengage from their E1 enzymes before E3-dependent ubiquitin and ubiquitin-like transfer

Eletr, ZM, Huang, DT, Duda, DM, Schulman, BA, Kuhlman, B

Nat. Struct. Mol. Biol. 2005
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