UBA1 conjugates ubiquitin to UBA1 in the cytosol

Stable Identifier
R-HSA-8852133
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

Activation of ubiquitin by UBA1 proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA1, and adenylation of a second molecule of ubiquitin. In the second step, the thiol group of an internal cysteine residue of UBA1 attacks the acyl-adenyl bond of the C-terminal glycine of adenylated ubiquitin, resulting in a thioester bond between the cysteine residue and the C-terminal glycine of ubiquitin (Jin et al. 2007, inferred from the rabbit homologue in Haas et al. 1982, Hershko et al. 1983, reviewed in Groettrup et al. 2008).

Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
ubiquitin activating enzyme activity of UBA1:ubiquitin adenylate [cytosol]
Physical Entity
Activity
Orthologous Events