AAK1 phosphorylates AP-2 mu subunit at T156

Stable Identifier
R-HSA-8856813
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

AAK1 is a serine-threonine kinase that phosphorylates T156 of the AP2 mu2 subunit (Olusanya et al, 2001; Conner et al, 2002; Conner et al, 2003). This phosphorylation is thought to stabilize the open conformation of the AP-2 complex, exposing the cargo-binding sites and promoting cargo capture (Ricotta et al, 2002). AAK1 kinase activity is stimulated by interaction with clathrin (Conner et al, 2003; Henderson et al, 2007).

Literature References
PubMed ID Title Journal Year
11516654 Phosphorylation of threonine 156 of the mu2 subunit of the AP2 complex is essential for endocytosis in vitro and in vivo

Olusanya, O, Andrews, PD, Swedlow, JR, Smythe, E

Curr. Biol. 2001
11877457 Phosphorylation of the AP2 mu subunit by AAK1 mediates high affinity binding to membrane protein sorting signals

Ricotta, D, Conner, SD, Schmid, SL, von Figura, K, Höning, S

J. Cell Biol. 2002
17494869 A novel AAK1 splice variant functions at multiple steps of the endocytic pathway

Henderson, DM, Conner, SD

Mol. Biol. Cell 2007
14617351 AAK1-mediated micro2 phosphorylation is stimulated by assembled clathrin

Conner, SD, Schröter, T, Schmid, SL

Traffic 2003
11877461 Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis

Conner, SD, Schmid, SL

J. Cell Biol. 2002
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
protein serine/threonine kinase activity of PI(4,5)P2:AP-2:clathrin:FCHO1,2:ITSNs:EPS15:REPS1:SGIP1:NECAPs:AAK1 [plasma membrane]
Physical Entity
Activity
Orthologous Events
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Reviewed