Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol

Stable Identifier
R-HSA-8865050
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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As inferred from the homologous UBA1, activation of ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the third step, the UBA6-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA6 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA6 and one of which is adenylated and non-covalently bound to UBA6 (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008).

Literature References
PubMed ID Title Journal Year
18353650 Activating the ubiquitin family: UBA6 challenges the field

Groettrup, M, Pelzer, C, Schmidtke, G, Hofmann, K

Trends Biochem. Sci. 2008
17597759 Dual E1 activation systems for ubiquitin differentially regulate E2 enzyme charging

Jin, J, Li, X, Gygi, SP, Harper, JW

Nature 2007
17580310 UBE1L2, a novel E1 enzyme specific for ubiquitin

Pelzer, C, Kassner, I, Matentzoglu, K, Singh, RK, Wollscheid, HP, Scheffner, M, Schmidtke, G, Groettrup, M

J. Biol. Chem. 2007
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Catalyst Activity
Title
ubiquitin activating enzyme activity of Ub-Cys625-UBA6 [cytosol]
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Activity
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Created