Ub-Cys625-UBA6 adenylates ubiquitin in the cytosol

Stable Identifier
R-HSA-8865050
Type
Reaction
Species
Homo sapiens
Compartment
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Summation

As inferred from the homologous UBA1, activation of ubiquitin by UBA6 (UBE1L1) proceeds through 3 steps: adenylation of ubiquitin, conjugation of ubiquitin from adenyl-ubiquitin to an internal cysteine residue of UBA6, and adenylation of a second molecule of ubiquitin. In the third step, the UBA6-ubiquitin conjugate adenylates the C-terminal glycine of a second ubiquitin molecule. This results in UBA6 loaded with 2 ubiquitin molecules, one of which is conjugated to an internal cysteine residue of UBA6 and one of which is adenylated and non-covalently bound to UBA6 (Jin et al. 2007, Pelzer et al. 2007, reviewed in Groettrup et al. 2008).

Literature References
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
ubiquitin activating enzyme activity of Ub-Cys625-UBA6 [cytosol]
Physical Entity
Activity
Inferred From