Reactome: A Curated Pathway Database

SNX9 recruits components of the actin polymerizing machinery

Stable Identifier
Homo sapiens
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Actin polymerization is not absolutely required for clathrin-mediated endocytosis, and disruption of actin does not interfere with the early stages of clathrin-coated pit formation. Actin is required to complete vesicle formation under conditions of high membrane tension, such as on the apical side of polarized epithelial cell, while actin is dispensable for this process in the absence of membrane tension (Boulant et al, 2011). In cases where actin is required, it appears to be recruited late to the emerging clathrin-coated pit, just prior to or coincident with the recruitment of dynamin and vesicle scission (Taylor et al, 2011; Taylor et al, 2012; reviewed in McMahon and Boucrot, 2011). Recruitment of actin depends on the ARP2/3 complex, and cortactin or the neural Wiscott-Aldrich syndrome proteins WASL. These proteins, in turn, are recruited through interactions with N-BAR domain containing proteins such as SNX9 (Yarar et al, 2007; Shin et al, 2007; Shin et al, 2008; Ferguson et al, 2009; reviewed in Lundmark and Carlsson, 2009; McMahon and Boucrot, 2011).
HIP1 and HIP1R are additional components of the late clathrin-coated pit that interact with clathrin and AP-2 and may contribute to actin nucleation (Waelter et al, 2001; Mishra et al, 2001;Metzler et al, 2001; Legendre-Guillemin et al, 2002; Wilbur et al, 2008; Taylor et al, 2011).

Literature References
PubMed ID Title Journal Year
11889126 HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain J. Biol. Chem. 2002
21779028 Molecular mechanism and physiological functions of clathrin-mediated endocytosis Nat. Rev. Mol. Cell Biol. 2011
21841790 Actin dynamics counteract membrane tension during clathrin-mediated endocytosis Nat. Cell Biol. 2011
20059951 Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits Dev. Cell 2009
18790740 Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain J. Biol. Chem. 2008
17681954 Sorting nexin 9 interacts with dynamin 1 and N-WASP and coordinates synaptic vesicle endocytosis J. Biol. Chem. 2007
11577110 Clathrin- and AP-2-binding sites in HIP1 uncover a general assembly role for endocytic accessory proteins J. Biol. Chem. 2001
11532990 The huntingtin interacting protein HIP1 is a clathrin and alpha-adaptin-binding protein involved in receptor-mediated endocytosis Hum. Mol. Genet. 2001
11517213 HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2 J. Biol. Chem. 2001
22505844 A feedback loop between dynamin and actin recruitment during clathrin-mediated endocytosis PLoS Biol. 2012
21445324 A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis PLoS Biol. 2011
18388313 SNX9 regulates tubular invagination of the plasma membrane through interaction with actin cytoskeleton and dynamin 2 J. Cell. Sci. 2008
19092055 SNX9 - a prelude to vesicle release J. Cell. Sci. 2009
17609109 SNX9 couples actin assembly to phosphoinositide signals and is required for membrane remodeling during endocytosis Dev. Cell 2007
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