Reactome: A Curated Pathway Database

RAB8 GEFs exchange GTP for GDP on RAB8

Stable Identifier
Homo sapiens
Locations in the PathwayBrowser

RAB8 is involved in trafficking from the trans-Golgi network (TGN) to the plasma membrane, contributes, with RAB10, 13 and 14 to GLUT4 transport, and plays a role in the formation of the primary cilium (Huber et al, 1993; Ishikura et al, 2008; Sun et al, 2010; Nachury et al, 2007; Yoshimura et al, 2007; reviewed in Hoffman and Elmendorf, 2011; Reiter et al, 2012). RAB8 also plays a role in regulating G2/M transition in complex with Optineurin (OPTN) (Kachaner et al, 2012).

RAB3IP is the best characterized GEF for RAB8A, with established roles in ciliogenesis (Hatulla et al, 2002; Knodler et al, 2010; Westlake et al, 2012; Wang et al, 2012; Feng et al, 2012; reviewed in Sung and Leroux, 2013). Other potential RAB8 GEFs include RAB3IPL and DENND1C (Yoshimura et al, 2010; reviewed in Ishida et al, 2016). Interaction of RAB8 with its GEFs displaces the GDI or CHM protein that keeps the inactive RAB:GDP soluble in the cytosol and promotes membrane association of RAB8 (reviewed in Wandinger-Ness and Zerial, 2014; Ishida et al, 2016)

Literature References
PubMed ID Title Journal Year
22653444 The base of the cilium: roles for transition fibres and the transition zone in ciliary formation, maintenance and compartmentalization EMBO Rep. 2012
18701652 Muscle cells engage Rab8A and myosin Vb in insulin-dependent GLUT4 translocation Am J Physiol Cell Physiol 2008
20937701 Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors J. Cell Biol. 2010
21041651 Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells Proc Natl Acad Sci U S A 2010
20308558 Coordination of Rab8 and Rab11 in primary ciliogenesis Proc. Natl. Acad. Sci. U.S.A. 2010
22983554 The Arf GAP ASAP1 provides a platform to regulate Arf4- and Rab11-Rab8-mediated ciliary receptor targeting EMBO J. 2012
17574030 A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis Cell 2007
21216617 Signaling, cytoskeletal and membrane mechanisms regulating GLUT4 exocytosis Trends Endocrinol Metab 2011
8408203 Rab8, a small GTPase involved in vesicular traffic between the TGN and the basolateral plasma membrane J. Cell Biol. 1993
17208202 Rabs 8A and 14 are targets of the insulin-regulated Rab-GAP AS160 regulating GLUT4 traffic in muscle cells Biochem Biophys Res Commun 2007
24296415 The roles of evolutionarily conserved functional modules in cilia-related trafficking Nat. Cell Biol. 2013
17646400 Functional dissection of Rab GTPases involved in primary cilium formation J. Cell Biol. 2007
12221131 A Rab8-specific GDP/GTP exchange factor is involved in actin remodeling and polarized membrane transport Mol. Biol. Cell 2002
22365832 Plk1-dependent phosphorylation of optineurin provides a negative feedback mechanism for mitotic progression Mol. Cell 2012
25341920 Rab proteins and the compartmentalization of the endosomal system Cold Spring Harb Perspect Biol 2014
21273506 Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome Proc. Natl. Acad. Sci. U.S.A. 2011
22433857 A Rab8 guanine nucleotide exchange factor-effector interaction network regulates primary ciliogenesis J. Biol. Chem. 2012
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases Cell Struct. Funct. 2016
Participant Of
This entity is regulated by:
Title Physical Entity Activity
Rab guanyl-nucleotide exchange factor activity of RAB8 GEFs [cytosol] RAB8 GEFs [cytosol] Rab guanyl-nucleotide exchange factor activity (0017112)
Orthologous Events