Reactome: A Curated Pathway Database

DENND1s exchange GTP for GDP on RAB35

Stable Identifier
Homo sapiens
Locations in the PathwayBrowser

RAB35 localizes to the plasma membrane, to clathrin-coated vesicles and to the endosome where it plays roles in recycling of endocytic cargo to the plasma membrane, in synaptic vesicle recycling and fusion of exosomes. RAB35 is also required for cytokinesis and contributes to the regulation of the actin cytoskeleton, and for GLUT4 translocation to the plasma membrane in response to insulin (Fuchs et al, 2007; Allaire et al, 2010; Kouranti et al, 2006; Dambournet et al, 2011; Rahajeng et al, 2012; Hsu et al. 2010; Uytterhoeven et al. 2011; Zhang et al. 2009; Davey et al, 2012; Humphrey et al, 2013; reviewed in Klinkert and Echard, 2016). RAB35 is activated by DENND1A, B and C (Sato et al, 2008; Allaire et al, 2010; Yoshimura et al, 2010; Marat and McPherson, 2010; Marat et al, 2012; reviewed in Marat et al, 2011; Ishida et al, 2016). Interaction of RAB35:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB35 with GDI and CHM proteins (Wu et al, 2011).

Literature References
PubMed ID Title Journal Year
16950109 Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis Curr. Biol. 2006
19729655 Rab35 controls actin bundling by recruiting fascin as an effector protein Science 2009
21458671 Loss of skywalker reveals synaptic endosomes as sorting stations for synaptic vesicle proteins Cell 2011
20937701 Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors J. Cell Biol. 2010
21330364 DENN domain proteins: regulators of Rab GTPases J. Biol. Chem. 2011
22072793 Connecdenn 3/DENND1C binds actin linking Rab35 activation to the actin cytoskeleton Mol. Biol. Cell 2012
27329675 Rab35 GTPase: a central regulator of phosphoinositides and F-actin in endocytic recycling and beyond Traffic 2016
18354496 Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein EMBO J. 2008
23684622 Dynamic adipocyte phosphoproteome reveals that Akt directly regulates mTORC2 Cell Metab. 2013
20159556 The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes Mol. Cell 2010
20404108 Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C J. Cell Biol. 2010
20154091 The connecdenn family, Rab35 guanine nucleotide exchange factors interfacing with the clathrin machinery J. Biol. Chem. 2010
17562788 Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways J. Cell Biol. 2007
22065758 Insights regarding guanine nucleotide exchange from the structure of a DENN-domain protein complexed with its Rab GTPase substrate Proc. Natl. Acad. Sci. U.S.A. 2011
21706022 Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis Nat. Cell Biol. 2011
22762500 TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes Traffic 2012
21951725 MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a Traffic 2012
27246931 Multiple Types of Guanine Nucleotide Exchange Factors (GEFs) for Rab Small GTPases Cell Struct. Funct. 2016
Participant Of
This entity is regulated by:
Title Physical Entity Activity
Rab guanyl-nucleotide exchange factor activity of RAB35 GEFs [cytosol] RAB35 GEFs [cytosol] Rab guanyl-nucleotide exchange factor activity (0017112)
Orthologous Events