RAB35 localizes to the plasma membrane, to clathrin-coated vesicles and to the endosome where it plays roles in recycling of endocytic cargo to the plasma membrane, in synaptic vesicle recycling and fusion of exosomes. RAB35 is also required for cytokinesis and contributes to the regulation of the actin cytoskeleton, and for GLUT4 translocation to the plasma membrane in response to insulin (Fuchs et al, 2007; Allaire et al, 2010; Kouranti et al, 2006; Dambournet et al, 2011; Rahajeng et al, 2012; Hsu et al. 2010; Uytterhoeven et al. 2011; Zhang et al. 2009; Davey et al, 2012; Humphrey et al, 2013; reviewed in Klinkert and Echard, 2016). RAB35 is activated by DENND1A, B and C (Sato et al, 2008; Allaire et al, 2010; Yoshimura et al, 2010; Marat and McPherson, 2010; Marat et al, 2012; reviewed in Marat et al, 2011; Ishida et al, 2016). Interaction of RAB35:GDP with its GEFs promotes release of GDP, allowing GTP to bind, and precludes the interaction of RAB35 with GDI and CHM proteins (Wu et al, 2011).