RUNX1 and GATA1 bind the promoter of the ITGA2B gene

Stable Identifier
R-HSA-8935740
Type
Reaction [binding]
Species
Homo sapiens
Compartment
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The RUNX1:CBFB complex can bind to the promoter of the ITGA2B (CD41) gene, encoding Integrin alpha-IIb, both in the absence and in the presence of PRMT1. PRMT1-mediated arginine-methylation significantly increases transcriptional activity of the RUNX1:CBFB complex at the ITGA2B promoter (Zhao et al. 2008). In addition to the RUNX1:CBFB complex, the complex of GATA1 and ZFPM1 (FOG1) (Freson et al. 2003) is also recruited to the ITGA2B promoter (Herglotz et al. 2013), likely through the interaction between GATA1 and RUNX1 (Elagib et al. 2003). The zinc finger domain of GATA1 is involved in binding to RUNX1 (Xu et al. 2006). Along with RUNX1 and GATA1, histone acetyltransferases p300 (EP300) and PCAF (KAT2B), as well as the WDR5-containing histone methyltransferase MLL complexes are also recruited to the ITGA2B promoter (Herglotz et al. 2013). Dimethylation of histone H3 on lysine residue K4 (K5 when taking into account the initiator methionine), known as the H3K4me2 mark, is characteristic of nucleosomes associated with megakaryocyte specific promoters, including the ITGA2B gene, prior to the onset of differentiation (Herglotz et al. 2013).

Literature References
PubMed ID Title Journal Year
18316480 Methylation of RUNX1 by PRMT1 abrogates SIN3A binding and potentiates its transcriptional activity

Zhao, X, Jankovic, V, Gural, A, Huang, G, Pardanani, A, Menendez, S, Zhang, J, Dunne, R, Xiao, A, Erdjument-Bromage, H, Allis, CD, Tempst, P, Nimer, SD

Genes Dev. 2008
12483298 Molecular cloning and characterization of the GATA1 cofactor human FOG1 and assessment of its binding to GATA1 proteins carrying D218 substitutions

Freson, K, Thys, C, Wittewrongel, C, Vermylen, J, Hoylaerts, MF, Van Geet, C

Hum. Genet. 2003
12576332 RUNX1 and GATA-1 coexpression and cooperation in megakaryocytic differentiation

Elagib, KE, Racke, FK, Mogass, M, Khetawat, R, Delehanty, LL, Goldfarb, AN

Blood 2003
22777353 Histone arginine methylation keeps RUNX1 target genes in an intermediate state

Herglotz, J, Kuvardina, ON, Kolodziej, S, Kumar, A, Hussong, H, Grez, M, Lausen, J

Oncogene 2013
16628190 Physical association of the patient-specific GATA1 mutants with RUNX1 in acute megakaryoblastic leukemia accompanying Down syndrome

Xu, G, Kanezaki, R, Toki, T, Watanabe, S, Takahashi, Y, Terui, K, Kitabayashi, I, Ito, E

Leukemia 2006
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