PXLP-KYAT1 dimer transaminates L-KYN to AP-DOBu

Stable Identifier
R-HSA-893596
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Synonyms
kynurenine + pyruvate => 4-(2-aminophenyl)-2,4-dioxobutanoic acid + alanine [CCBL1]
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Kynurenine-oxoglutarate transaminase 1 (KYAT1, aka CCBL1, KAT1) catalyzes the reaction of kynurenine (L-KYN) and pyruvate (PYR) to form 4-(2-aminophenyl)-2,4-dioxobutanoate (AP-DOBu) and alanine (L-Ala). The active form of KYAT1 is a homodimer with one molecule of pyridoxal phosphate (PXLP) bound to each monomer (Baran et al. 1994, Han et al. 2009, Rossi et al. 2004). The enzyme's cytosolic localization is inferred from recombinant protein overexpressed in transfected cells (Perry et al. 1995). The pH optimum observed for KYAT1 in vitro is 9.5 - 10.0, so its role in kynurenine metabolism in vivo is not clear (Baran et al. 1994).

Biochemical studies of KYAT1 activity in vitro (e.g. Baren et al. 1994) invariably measure kynurenic acid as the reaction product, not AP-DOBu, the product to be expected from transamination of kynurenine. The condensation of AP-DOBu and elimination of a water molecule to form kynurenic acid has not been demonstrated directly. As noted by Miller et al. (1953) discussing their characterization of a bacterial form of the enzyme, "The keto acid assumed to be formed prior to ring closure in the conversion of kynurenine to kynurenic acid has not yet been detected. In principle, such detection should be possible, since it is sufficiently stable to have been synthesized. It also remains to be established whether ring closure is spontaneous, enzymatic, or both. The formation of kynurenic acid from L-kynurenine by the L-amino acid oxidase of Neurospora suggests, however, that ring closure can be spontaneous, unless the somewhat improbable assumption is made that Neurospora filtrate contained the ring-closing enzyme."

The alpha keto acids indole-3-propionic acid (I3PROPA) and indole-3-lactic acid (I3LACT) are potent inhibitors of KYAT1 (Han et al. 2009).

Literature References
PubMed ID Title Journal Year
15364907 Crystal structure of human kynurenine aminotransferase I

Rossi, F, Han, Q, Li, J, Li, J, Rizzi, M

J Biol Chem 2004
8294935 Purification and characterization of kynurenine aminotransferase I from human brain

Baran, H, Okuno, E, Kido, R, Schwarcz, R

J Neurochem 1994
7883047 Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase

Perry, S, Harries, H, Scholfield, C, Lock, T, King, L, Gibson, G, Goldfarb, P

FEBS Lett 1995
19338303 Structural insight into the inhibition of human kynurenine aminotransferase I/glutamine transaminase K

Han, Q, Robinson, H, Cai, T, Tagle, DA, Li, J

J Med Chem 2009
13069505 The transamination of kynurenine

Miller, IL, Tsuchida, M, Adelberg, EA

J Biol Chem 1953
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
kynurenine-oxoglutarate transaminase activity of PXLP-KYAT1 dimer [cytosol]
Physical Entity
Activity
This event is regulated
Orthologous Events
Authored
Reviewed
Created