RNF20:RNF40 monoubiquitinates Histone H2B

Stable Identifier
R-HSA-8942101
Type
Reaction [omitted]
Species
Homo sapiens
Compartment
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The ubiquitin E3 ligase complex RNF20:RNF40 interacts with the PAF complex (Kim et al. 2009) that is associated with RNA polymerase II via WAC (Zhang and Yu 2011) at transcriptionally active genes (Zhe et al. 2005). RNF20:RNF40 monoubiquitinates nucleosomal histone H2B on lysine-120 (lysine-121 of the unprocessed histone H2B) using UBE2A,B:Ubiquitin as the ubiquitin donor (Zhu et al. 2005, Kim et al. 2009, Zhang and Yu 2011, Zhang et al. 2014, Dickson et al. 2016). Monoubiquitination of histone H2B leads to methylation of lysine-4 and lysine-79 of histone H3, marks of active chromatin (Zhu et al. 2005). Arsenite binds the RING domains of RNF20 and RNF40 and inhibits the ubiquitination of histone H2B (Zhang et al. 2014).

Literature References
PubMed ID Title Journal Year
16307923 Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation

Zhu, B, Zheng, Y, Pham, AD, Mandal, SS, Erdjument-Bromage, H, Tempst, P, Reinberg, D

Mol. Cell 2005
19410543 RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells

Kim, J, Guermah, M, McGinty, RK, Lee, JS, Tang, Z, Milne, TA, Shilatifard, A, Muir, TW, Roeder, RG

Cell 2009
27798111 The RING finger domain E3 ubiquitin ligases BRCA1 and the RNF20/RNF40 complex in global loss of the chromatin mark histone H2B monoubiquitination (H2Bub1) in cell line models and primary high-grade serous ovarian cancer

Dickson, KA, Cole, AJ, Gill, AJ, Clarkson, A, Gard, GB, Chou, A, Kennedy, CJ, Henderson, BR

Hum. Mol. Genet. 2016
25170678 Arsenite binds to the RING finger domains of RNF20-RNF40 histone E3 ubiquitin ligase and inhibits DNA double-strand break repair

Zhang, F, Paramasivam, M, Cai, Q, Dai, X, Wang, P, Lin, K, Song, J, Seidman, MM, Wang, Y

J. Am. Chem. Soc. 2014
21329877 WAC, a functional partner of RNF20/40, regulates histone H2B ubiquitination and gene transcription

Zhang, F, Yu, X

Mol. Cell 2011
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
ubiquitin protein ligase activity of PAF1c:WAC:RNF20:RNF40:Ub:UBE2A,B:HIST1H2B [nucleoplasm]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created