Reactome | IL12B dimerizes

IL12B dimerizes

Stable Identifier

R-HSA-8950456

Type

Reaction [transition]

Species

Homo sapiens

Compartment

endoplasmic reticulum lumen

Synonyms

Interleukin-12 subunit beta binds Interleukin-12 subunit beta forming Interleukin-12 beta homodimer, IL-12B binds IL-12B to for a IL12B homodimer

ReviewStatus

5/5

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Murine cells have been shown to produce Interleukin-12 homodimer (IL-12p80) (Gillessen et al. 1995). In a cell-free system porcine Interleukin 12 homodimer (IL-12p80) is formed from two subunits of Interleukin 12 subunit beta (IL12B, IL-12p40), stabilized by a disulfide link. The cysteine in position 197 of IL12B is required for homodimer formation. Prolyl 4-hydroxylase subunit beta (P4HB), in this article referred to as protein disulfide isomerase (PDI), can act as a chaperone to induce homodimer formation (Martens et al. 2000). Human cells do not produce IL-12p80 under normal conditions (Ling et al. 1995), when however expressed acts as an antagonist of Interleukin-12 signaling, competing with the Interleukin 12 heterodimer (IL12) for its receptor (Ling et al. 1995).

Literature References

PubMed ID	Title	Journal	Year
7527811	Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity Stern, AS, Hollfelder, K, Su, C, Hakimi, J, Gubler, U, Gately, MK, Lin, P, Pan, YC, Ling, P	J. Immunol.	1995
25259790	Recombinant p35 from bacteria can form Interleukin (IL-)12, but Not IL-35 Garbers, C, Plöhn, S, Lang, PA, Grötzinger, J, Lokau, J, Moll, JM, Aparicio-Siegmund, S, Scheller, J, Schröder, J, Grusdat, M, Rose-John, S	PLoS ONE	2014
11054122	Protein disulfide isomerase-mediated cell-free assembly of recombinant interleukin-12 p40 homodimers Vandenbroeck, K, Billiau, A, Alloza, I, Scott, CJ, Martens, E	Eur. J. Biochem.	2000