Transfer of NEDD8 to AcM-UBE2M

Stable Identifier
R-HSA-8951661
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

UBA3:NAE1 transfers NEDD8 to the catalytic cysteine residue of UBE2M. Because two of the three E1-E2 interaction interfaces are created by conformational states present in the doubly-NEDDylated NAE1, transfer of NEDD8 to UBE2M is thought to weaken the interaction between UBA3:NAE1 and UBE2M, contributing to UBE2M release (Walden et al, 2003; Huang et al, 2004; Hunag et al, 2005; Huang et al, 2007).

Literature References
PubMed ID Title Journal Year
17220875 Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity

Huang, DT, Hunt, HW, Zhuang, M, Ohi, MD, Holton, JM, Schulman, BA

Nature 2007
14690597 The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1

Walden, H, Podgorski, MS, Huang, DT, Miller, DW, Howard, RJ, Minor, DL, Holton, JM, Schulman, BA

Mol. Cell 2003
15694336 Structural basis for recruitment of Ubc12 by an E2 binding domain in NEDD8's E1

Huang, DT, Paydar, A, Zhuang, M, Waddell, MB, Holton, JM, Schulman, BA

Mol. Cell 2005
15361859 A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8

Huang, DT, Miller, DW, Mathew, R, Cassell, R, Holton, JM, Roussel, MF, Schulman, BA

Nat. Struct. Mol. Biol. 2004
Participants
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
NEDD8 transferase activity of AcM-UBE2M:NEDD8-UBA3:NAE1:NEDD8 [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed