AcM-UBE2M transfers NEDD8 to CRL1 E3 ubiquitin ligase complex

Stable Identifier
R-HSA-8952618
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

UBE2M transfers NEDD8 to lysine 720 of CUL1 in the CRL E3 ubiquitin ligase complex (Hori et al, 1999; Duda et al, 2008). Neddylation increases the ubiquitination activity of the E3 complex towards its targets, and prevents binding of the CUL1 complex with the CAND1 inhibitor (Hori et al, 1999; Goldenberg et al, 2004; Duda et al, 2008; Kelsall et al, 2013; Scott et al, 2016). Targets of CUL1 RING complexes include a variety of cellular proteins including regulators of transcription and cell cycle progression, among others (reviewed in Lipkowitz and Weissman, 2011). CRL1 complexes are also hijacked by viruses, redirecting the ubiquitin ligase complex to target host proteins (reviewed in Mahon et al, 2014).

Literature References
PubMed ID Title Journal Year
10597293 Covalent modification of all members of human cullin family proteins by NEDD8

Hori, T, Osaka, F, Chiba, T, Miyamoto, C, Okabayashi, K, Shimbara, N, Kato, S, Tanaka, K

Oncogene 1999
25314029 Cullin E3 ligases and their rewiring by viral factors

Mahon, C, Krogan, NJ, Craik, CS, Pick, E

Biomolecules 2014
27565346 Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation

Scott, DC, Rhee, DY, Duda, DM, Kelsall, IR, Olszewski, JL, Paulo, JA, de Jong, A, Ovaa, H, Alpi, AF, Harper, JW, Schulman, BA

Cell 2016
21863050 RINGs of good and evil: RING finger ubiquitin ligases at the crossroads of tumour suppression and oncogenesis

Lipkowitz, S, Weissman, AM

Nat. Rev. Cancer 2011
24076655 TRIAD1 and HHARI bind to and are activated by distinct neddylated Cullin-RING ligase complexes

Kelsall, IR, Duda, DM, Olszewski, JL, Hofmann, K, Knebel, A, Langevin, F, Wood, N, Wightman, M, Schulman, BA, Alpi, AF

EMBO J. 2013
15537541 Structure of the Cand1-Cul1-Roc1 complex reveals regulatory mechanisms for the assembly of the multisubunit cullin-dependent ubiquitin ligases

Goldenberg, SJ, Cascio, TC, Shumway, SD, Garbutt, KC, Liu, J, Xiong, Y, Zheng, N

Cell 2004
18805092 Structural insights into NEDD8 activation of cullin-RING ligases: conformational control of conjugation

Duda, DM, Borg, LA, Scott, DC, Hunt, HW, Hammel, M, Schulman, BA

Cell 2008
Participants
Participant Of
hasEvent
Catalyst Activity
Catalyst Activity
Title
NEDD8 transferase activity of NEDD8-AcM-UBE2M:CRL1 E3 ubiquitin ligase:COMMDs:CCDC22:DCUN1Ds [cytosol]
Physical Entity
Activity
This entity is regulated by
Orthologous Events
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