Based on studies in mice, ROBO1, activated by SLIT1 binding, forms a complex with FLRT3. This interaction involves the intracellular domains of FLRT3 and ROBO1. FLRT3 is a member of the fibronectin leucine-rich repeat transmembrane protein family. The interaction of FLRT3 and ROBO1 in the presence of SLIT1 increases Netrin-1 attraction of thalamocortical axons by increasing the amount of DCC receptors at the plasma membrane via an unknown mechanism that may involve PKA activation (Leyva-Diaz et al. 2014).