SPO11 hydrolyzes DNA forming double-strand breaks

Stable Identifier
R-HSA-912368
Type
Reaction [transition]
Species
Homo sapiens
Compartment
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The gene encoding SPO11 shares sequence similarity to TopoVI, a type II topoisomerase. SPO11 dimers cleave both strands of DNA. Each subunit of the dimer remains covalently attached to the 5' end of one strand of DNA via a phosphodiester linkage to a conserved tyrosine residue of SPO11. In addition to SPO11, work from budding yeast has shown a total of 7 proteins essential for double strand break formation. The mammalian ortholog of Mei4 (S. cerevisiae) as well as a mammalian-specific gene called Mei1 are essential to formation of meiotic double strand breaks.

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Catalyst Activity
Catalyst Activity
Title
endodeoxyribonuclease activity, producing 5'-phosphomonoesters of SPO11 Dimer [nucleoplasm]
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Activity
Inferred From
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