Tail-anchored protein:ASNA1:ADP binds WRB:CAMLG at the endoplasmic reticulum membrane

Stable Identifier
R-HSA-9609899
Type
Reaction [binding]
Species
Homo sapiens
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A tail-anchored protein bound to ASNA1 dimer (TRC40 dimer) in the cytosol (Stefanovic and Hegde 2007, Favaloro et al. 2008, Favaloro et al. 2010) docks at the WRB:CAMLG complex located in the endoplasmic reticulum membrane (Vilardi et al. 2011, Yamamoto and Sakisaka 2012, Vilardi et al. 2014, Colombo et al. 2016, Pfaff et al. 2016, Vogl et al. 2016). The cytoplasmic coiled coil domain of WRB and the cytoplasmic N-terminal domain of CAMLG compete for binding to ASNA1 (Vilardi et al. 2011, Yamamoto and Sakisaka 2012). As inferred from yeast homologs, ASNA1 hydrolyzes bound ATP at some point, resulting in a change of conformation of ASNA1. This may transfer the tail-anchored protein to WRB:CAMLG, which functions as an insertase.

Literature References
PubMed ID Title Journal Year
17382883 Identification of a targeting factor for posttranslational membrane protein insertion into the ER

Stefanovic, S, Hegde, RS

Cell 2007
18477612 Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins

Favaloro, V, Spasic, M, Schwappach, B, Dobberstein, B

J. Cell. Sci. 2008
21444755 WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane

Vilardi, F, Lorenz, H, Dobberstein, B

J. Cell. Sci. 2011
24392163 WRB and CAML are necessary and sufficient to mediate tail-anchored protein targeting to the ER membrane

Vilardi, F, Stephan, M, Clancy, A, Janshoff, A, Schwappach, B

PLoS ONE 2014
20375064 Asna1/TRC40-mediated membrane insertion of tail-anchored proteins

Favaloro, V, Vilardi, F, Schlecht, R, Mayer, MP, Dobberstein, B

J. Cell. Sci. 2010
26675233 Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane

Pfaff, J, Rivera Monroy, J, Jamieson, C, Rajanala, K, Vilardi, F, Schwappach, B, Kehlenbach, RH

J. Cell. Sci. 2016
27226539 Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR

Colombo, SF, Cardani, S, Maroli, A, Vitiello, A, Soffientini, P, Crespi, A, Bram, RF, Benfante, R, Borgese, N

J. Biol. Chem. 2016
23041287 Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells

Yamamoto, Y, Sakisaka, T

Mol. Cell 2012
27458190 Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing

Vogl, C, Panou, I, Yamanbaeva, G, Wichmann, C, Mangosing, SJ, Vilardi, F, Indzhykulian, AA, Pangršič, T, Santarelli, R, Rodriguez-Ballesteros, M, Weber, T, Jung, S, Cardenas, E, Wu, X, Wojcik, SM, Kwan, KY, del Castillo, I, Schwappach, B, Strenzke, N, Corey, DP, Lin, SY, Moser, T

EMBO J. 2016
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