Tail-anchored protein:ASNA1:ADP:WRB:CAMLG dissociates yielding Tail-anchored protein in the endoplasmic reticulum membrane

Stable Identifier
R-HSA-9609917
Type
Reaction [dissociation]
Species
Homo sapiens
Compartment
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A tail-anchored protein bound to ASNA1 (TRC40) (Stefanovic and Hegde 2007, Favaloro et al. 2008, Favaloro et al. 2010) docked at the WRB:CAMLG complex (also known as the WRB:CAML complex) located in the endoplasmic reticulum membrane is released into the ER membrane in an incompletely characterized reaction (Vilardi et al. 2011, Yamamoto and Sakisaka 2012, Vilardi et al. 2014, Colombo et al. 2016, Pfaff et al. 2016, Vogl et al. 2016). Experiments with yeast homologs (GET1 is the homologue of WRB and GET2 is the homologue of CAMLG) indicate that docking of ASNA1:ADP with WRB:CAMLG results in a conformational change in ASNA1 that causes release of the tail-anchored protein to WRB:CAMLG and then into the membrane (inferred from yeast homologs), ADP dissociates from ASNA1 at some point during the reaction. Exchange of ADP for ATP by ASNA1 then recycles ASNA1 back into the cytosol (inferred from yeast homologs).

Literature References
PubMed ID Title Journal Year
17382883 Identification of a targeting factor for posttranslational membrane protein insertion into the ER

Stefanovic, S, Hegde, RS

Cell 2007
18477612 Distinct targeting pathways for the membrane insertion of tail-anchored (TA) proteins

Favaloro, V, Spasic, M, Schwappach, B, Dobberstein, B

J. Cell. Sci. 2008
21444755 WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane

Vilardi, F, Lorenz, H, Dobberstein, B

J. Cell. Sci. 2011
20375064 Asna1/TRC40-mediated membrane insertion of tail-anchored proteins

Favaloro, V, Vilardi, F, Schlecht, R, Mayer, MP, Dobberstein, B

J. Cell. Sci. 2010
26675233 Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane

Pfaff, J, Rivera Monroy, J, Jamieson, C, Rajanala, K, Vilardi, F, Schwappach, B, Kehlenbach, RH

J. Cell. Sci. 2016
27226539 Tail-anchored Protein Insertion in Mammals: FUNCTION AND RECIPROCAL INTERACTIONS OF THE TWO SUBUNITS OF THE TRC40 RECEPTOR

Colombo, SF, Cardani, S, Maroli, A, Vitiello, A, Soffientini, P, Crespi, A, Bram, RF, Benfante, R, Borgese, N

J. Biol. Chem. 2016
23041287 Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells

Yamamoto, Y, Sakisaka, T

Mol. Cell 2012
27458190 Tryptophan-rich basic protein (WRB) mediates insertion of the tail-anchored protein otoferlin and is required for hair cell exocytosis and hearing

Vogl, C, Panou, I, Yamanbaeva, G, Wichmann, C, Mangosing, SJ, Vilardi, F, Indzhykulian, AA, Pangršič, T, Santarelli, R, Rodriguez-Ballesteros, M, Weber, T, Jung, S, Cardenas, E, Wu, X, Wojcik, SM, Kwan, KY, del Castillo, I, Schwappach, B, Strenzke, N, Corey, DP, Lin, SY, Moser, T

EMBO J. 2016
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