N-glycan trimming and elongation in the cis-Golgi

Stable Identifier
R-HSA-964739
Type
Pathway
Species
Homo sapiens
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this pathway in the Pathway Browser

After the transport of the glycoprotein to the cis-Golgi, the pathway of N-glycosylation bifurcates. Some N-glycans can be moved to subsequent steps of the secretory pathway without further modifications, or alternatively, with the removal of a few mannoses (Oligo Mannoses pathway). In yeast and other unicellular species, a series of mannose residues are added (High Mannoses pathway). The presence of this modification is a major obstacle to the production of pharmaceutical drugs in yeast, where the HighMannose pathway must be inhibited or modified in order to avoid the presence of high mannose xenoglycans.
The first N-glycan modification step is the trimming of up to four mannoses by one of three mannosidase enzymes. Moreover, Glycoproteins that have not entered in the Calnexin/Calreticulin cycle or that have not had their glucose residues trimmed earlier in the ER, can enter the main pathway here due to the existence to an alternative route catalyzed by the enzyme Endomannosidase I (Schachter, 2000; Stanley et al, 2009)

Literature References
PubMed ID Title Journal Year
11421343 The joys of HexNAc. The synthesis and function of N- and O-glycan branches

Schachter, H

Glycoconj J 2000
  N-Glycans

Stanley, P, Stanley, P, Schachter, H, Taniguchi, N, Varki, A, Cummings, RD, Esko, JD, Freeze, HH, Bertozzi, CR, Hart, GW, Etzler, ME

  2009
Participants
Participant Of
Orthologous Events
Authored
Reviewed
Created