SARS-CoV-1 M protein interacts with TBK1/IKBKE

Stable Identifier
R-HSA-9710988
Type
Reaction [binding]
Species
Homo sapiens
Related Species
Human SARS coronavirus
Compartment
Golgi membrane, cytosol
ReviewStatus
5/5
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SARS-CoV-1 M protein interacts with TBK1/IKBKE
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Severe acute respiratory syndrome coronavirus type 1 (SARS‑CoV‑1) M protein is a glycosylated structural protein with three transmembrane (TM) domains. M protein predominantly localizes to the Golgi complex and is essential for the assembly of viral particles. SARS‑CoV‑1 M protein suppresses the production of type I IFNs in human embryonic kidney 293 cells (HEK293 cells) expressing RIG‑I (DDX58), MDA5 (IFIH1), TRAF3, TBK1 and IKKε (IKBKE) proteins (Siu KL et al. 2009; 2014). IFN antagonism was mediated by the N‑terminal TM1 domain of M protein (amino acids 1–38), which targets M protein to the Golgi complex (Siu KL et al. 2014). Co‑immunoprecipitation analysis revealed that TM1 of M protein interacted with TRAF3, RIG‑I (DDX58), TBK1 and IKKε (IKBKE) (Siu KL et al. 2014) in HEK293 expressing tagged proteins (Siu KL et al. 2014). SARS‑CoV‑1 M protein is thought to prevent the formation of the TRAF3:TANK:TBK1/IKBKE complex and thereby inhibit TBK1/IKBKE‑dependent activation of IRF3/IRF7 transcription factors downstream of MAVS (Siu KL et al. 2009; 2014). The Reactome event shows binding of SARS‑CoV‑1 M protein to TBK1/IKBKE.
Literature References
PubMed ID Title Journal Year
19380580 Severe acute respiratory syndrome coronavirus M protein inhibits type I interferon production by impeding the formation of TRAF3.TANK.TBK1/IKKepsilon complex

Yuen, KY, Poon, VK, Jin, DY, Ng, MH, Siu, KL, Kok, KH, Zheng, BJ

J. Biol. Chem. 2009
24509444 Suppression of innate antiviral response by severe acute respiratory syndrome coronavirus M protein is mediated through the first transmembrane domain

Chiu-Yat Woo, P, Jin, DY, Chan, CP, Siu, KL, Kok, KH

Cell. Mol. Immunol. 2014
Participants
Input
Output
Participates
as an event of
Disease
Name Identifier Synonyms
severe acute respiratory syndrome DOID:2945 SARS-CoV infection, SARS
Authored
Shamovsky, V  (2020-06-25)
Reviewed
D'Eustachio, P  (2021-01-26)
Created
Shamovsky, V  (2021-01-06)
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