miR-200c-3p binds CDH11 mRNA

Stable Identifier
R-HSA-9759950
Type
Reaction [binding]
Species
Homo sapiens
Compartment
cytosol
ReviewStatus
5/5
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miR-200c-3p binds CDH11 mRNA
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Based on the luciferase reporter assay, microRNA miR-200c-3p binds to the 3'UTR of CDH11 mRNA, which results in decreased levels of CDH11 mRNA. Upregulation of miR-200C-3p is associated with CDH11 mRNA downregulation in colonic epithelial cells, which may play a role in the pathogenesis of ulcerative colitis (Van der Goten et al. 2014). Downregulation of miR-200c-3p has been reported in invasive breast cancer cell lines where it is associated with upregulation of CDH11 mRNA (Luo et al. 2013).

As the 3'UTR is identical in the two human CDH11 splicing isoforms, both mRNA isoforms are shown as targets of miR-200c-3p, with the short variant annotated as a set candidate. Since miR-200c-3p reduces CDH11 mRNA level, the miR-200c-3p endonucleolytic RISC is annotated as a set member of the miR-200c-3p RISC set, while the miR-200c-3p nonendonucleolytic RISC is annotated as a set candidate. (Footnote: Two versions of RNA-induced silencing complex, RISC, exist: endonuleolytic and nonendonucleolytic. Endonucleolytic RISC cleaves the target mRNA, leading to reduction in both mRNA and protein levels. Nonendonuleolytic RISC does not cleave the target mRNA, but still interferes with translation, leading to reduced protein level of the target gene, but no change in the mRNA level. If the study Reactome is citing shows reduction of mRNA level off the target in the presence of miRNA, the involvement of an endonucleolytic RISC is certain. Hence, the endonucleolytic RISC is annotated either on its own if the involvement of a nonendonucleolytic RISC can be excluded, or as a set member if the involvement of a nonendonucleolytic RISC cannot be excluded. In the latter case, which is the most frequent scenario encountered in published studies, the corresponding nonendonucleolytic RISC is annotated as a candidate. If the experimental results show decreased protein level of the miRNA target gene but no change in the mRNA level, than only a nonendonucleolytic RISC is annotated. Please refer to the pathway "Gene Silencing by RNA" for additional details.)
Literature References
PubMed ID Title Journal Year
23497265 A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells

Liu, J, Huang, S, Wilson, JM, Pei, L, Hawthorn, L, Harvel, N, Luo, D, Shi, H

J Transl Med 2013
25546151 Integrated miRNA and mRNA expression profiling in inflamed colon of patients with ulcerative colitis

Van Assche, G, Rutgeerts, P, Wollants, WJ, Vermeire, S, Schuit, F, Ferrante, M, Lemaire, K, Van der Goten, J, Arijs, I, de Hertogh, G, Vanhove, W, Van Lommel, L, Machiels, K, De Preter, V

PLoS One 2014
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Authored
Orlic-Milacic, M  (2022-06-02)
Reviewed
Brasch, J  (2023-04-18)
Created
Orlic-Milacic, M  (2021-12-14)
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