Transfer of ubiquitin from E1 to E2

Stable Identifier
R-HSA-983152
Type
Reaction
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
Summation

Activated ubiquitin is transferred from E1 to the active site cystine of ubiquitin conjugating enzymes (E2s) via a trans-esterification reaction. E2s catalyze covalent attachment of ubiquitin to target proteins. They all share an active-site ubiquitin-binding cysteine residue and are distinguished by the presence of a ubiquitin-conjugating catalytic (UBC) fold required for binding of distinct ubiquitin ligases or E3s. Once conjugated to ubiquitin, the E2 molecule binds one of several E3s (Glickman et al. 2002).

Literature References
PubMed ID Title Journal Year
19851334 Building ubiquitin chains: E2 enzymes at work

Ye, Y, Rape, M

Nat Rev Mol Cell Biol 2009
19940261 The family of ubiquitin-conjugating enzymes (E2s): deciding between life and death of proteins

van Wijk, SJ, Timmers, HT

FASEB J 2010
19452197 What was the set of ubiquitin and ubiquitin-like conjugating enzymes in the eukaryote common ancestor?

Michelle, C, Vourc'h, P, Mignon, L, Andres, CR

J Mol Evol 2009
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
ubiquitin binding activity of E2 Ubiquitin-conjugating enzyme [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed