E1 mediated ubiquitin activation

Stable Identifier
R-HSA-983153
Type
Reaction [transition]
Species
Homo sapiens
Compartment
Locations in the PathwayBrowser
General
SVG |   | PPTX  | SBGN
Click the image above or here to open this reaction in the Pathway Browser
The layout of this reaction may differ from that in the pathway view due to the constraints in pathway layout

Ubiquitin is activated in an ATP-dependent manner, catalyzed by E1 ubiquitin-activating enzymes. In the first step of ubiquitin activation, the E1 enzyme binds ATP, Mg+2 and ubiquitin, and catalyses ubiquitin C-terminal acyl-adenylation (Ubiquitin-AMP). In the second step, the catalytic cystine in the E1 attacks the ubiquitin-adenylate (Ub-AMP) to form the activated ubiquitin-E1 thioester-bonded complex and an AMP leaving group. The intermediate reaction involving the formation of the ubiquitin-AMP complex is not represented here.

Literature References
PubMed ID Title Journal Year
19352404 Ubiquitin-like protein activation by E1 enzymes: the apex for downstream signalling pathways

Schulman, BA, Harper, JW

Nat Rev Mol Cell Biol 2009
16595681 Pleiotropic effects of ATP.Mg2+ binding in the catalytic cycle of ubiquitin-activating enzyme

Tokgöz, Z, Bohnsack, RN, Haas, AL

J Biol Chem 2006
18662542 Structural insights into E1-catalyzed ubiquitin activation and transfer to conjugating enzymes

Lee, I, Schindelin, H

Cell 2008
Participants
Participant Of
Catalyst Activity
Catalyst Activity
Title
ubiquitin-protein transferase activity of Ubiquitin activating E1 enzymes [cytosol]
Physical Entity
Activity
Orthologous Events
Authored
Reviewed
Created
Cite Us!