Reactome | PLK1 phosphorylates FIRMM at S744

PLK1 phosphorylates FIRMM at S744

Stable Identifier

R-HSA-9853369

Type

Reaction [transition]

Species

Homo sapiens

Compartment

cytosol

ReviewStatus

5/5

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PLK1 phosphorylates FIRRM (also known as Apolo1 or FLIP or C1orf112) on a conserved serine residue S744, in the PP1 interaction motif KVVSF, thus inhibiting association of FIRRM with PPP1CC. PPP1CC not bound to FIRRM is able to dephosphorylate PLK1 on threonine residue T210, thus inactivating it. As PLK1 also phosphorylates FIRRM on S43, which positively affects FIRRM association with PLK1 and promotes PLK1 kinase activity at kinetochores, it is plausible that PLK1-mediated phosphorylation of FIRRM at S744 succeeds the phosphorylation at S43 and creates a negative feedback loop. This negative feedback loop involving FIRRM, PLK1, and PPP1CC is proposed to fine-tune PLK1 catalytic activity at kinetochores during prometaphase and enable timely segregation of replicated chromosomes during metaphase (Xu et al. 2021).

Literature References

PubMed ID	Title	Journal	Year
34260926	Feedback control of PLK1 by Apolo1 ensures accurate chromosome segregation Sun, B, Tian, R, Poser, I, Garba, F, Li, L, Liu, X, Green, H, Chu, L, Ge, Y, Dou, Z, Lu, J, Pan, W, Yuan, X, Liu, D, Wang, D, Mullen, M, Duan, W, Duan, H, Ali, M, Hyman, A, Yao, X, Xu, L	Cell Rep	2021