Reactome | Pld6 dimer cleaves primary piRNA transcript to pre-piRNA

Pld6 dimer cleaves primary piRNA transcript to pre-piRNA

Stable Identifier

R-MMU-5605262

Type

Reaction [transition]

Species

Mus musculus

Compartment

cytosol, mitochondrial outer membrane

ReviewStatus

5/5

General

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SVG | | PPTX | SBGN

Pld6 dimer cleaves primary piRNA transcript to pre-piRNA

Mouse PLD6 (MitoPLD) located on the cytoplasmic face of the mitochondrial outer membrane (Choi et al. 2006, Watanabe et al. 2011) makes the first endonucleolytic cleavage of primary piRNA transcripts (Ipsaro et al. 2012, Nishimasu et al. 2012). The cleavage yields a 5' phosphate and a 3' hydroxyl. Cleavage is believed to precede loading into PIWIL1 (MIWI).

Literature References

PubMed ID	Title	Journal	Year
21397847	MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline Totoki, Y, Lin, H, Kuramochi-Miyagawa, S, Watanabe, T, Toyoda, A, Chuma, S, Fujiyama, A, Sasaki, H, Hoki, Y, Yamamoto, Y, Nakano, T, Noce, T, Nakatsuji, N, Shibata, T, Sado, T	Dev. Cell	2011
23064227	The structural biochemistry of Zucchini implicates it as a nuclease in piRNA biogenesis Hannon, GJ, Knott, SR, Joshua-Tor, L, Haase, AD, Ipsaro, JJ	Nature	2012
17028579	A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis Jenkins, GM, Huang, P, Schiller, J, Chan, DC, Frohman, MA, Choi, SY	Nat. Cell Biol.	2006
23064230	Structure and function of Zucchini endoribonuclease in piRNA biogenesis Fukuhara, S, Nakanaga, K, Siomi, H, Matsumoto, N, Kamatani, MK, Saito, K, Ishizu, H, Siomi, MC, Bonnefond, L, Nishimasu, H, Nureki, O, Aoki, J, Ishitani, R, Nishizawa, T	Nature	2012