Hoxd3 chromatin is activated

Stable Identifier
Reaction [BlackBoxEvent]
Mus musculus

Retinoic acid induces expression of Hoxd3 in embryos (Folberg et al. 1999) and fetal cortical neurons (Chai et al. 2009), but it is unknown if the effect is direct or indirect. The retinoid receptor Rarb is not responsible for the inducibility in embryos (Folberg et al. 1999). Hoxd3 is expressed in rhombomeres 5,6, and 7 (r5-7) (Tan et al. 1996, Gaufo et al. 2003) and in more caudal regions: the spinal cord, dorsal root ganglia, first cervical vertebrae, thyroid gland, kidney tublules, esophagus, stomach, and intestines (Tan et al. 1996).
In mouse embryonic stem cells treated with retinoic acid Hoxd3 chromatin is activated by loss of methylation at lysine-27 of histone H3 (H3K27me3) and loss of Polycomb repressive complex 2 (PRC2) (Williamson et al. 2012, Mazzoni et al. 2013). In human fibroblasts, the histone demethylase KDM6A participates in removing H3K27me3. The Mll2 complex methylates H3K4 at Hoxd3 in fibroblasts (Wang et al. 2009).

Literature References
PubMed ID Title Journal Year
12954718 Hox3 genes coordinate mechanisms of genetic suppression and activation in the generation of branchial and somatic motoneurons

Gaufo, GO, Thomas, KR, Capecchi, MR

Development 2003
19703992 Global analysis of H3K4 methylation defines MLL family member targets and points to a role for MLL1-mediated H3K4 methylation in the regulation of transcriptional initiation by RNA polymerase II

Wang, P, Lin, C, Smith, ER, Guo, H, Sanderson, BW, Wu, M, Gogol, M, Alexander, T, Seidel, C, Wiedemann, LM, Ge, K, Krumlauf, R, Shilatifard, A

Mol. Cell. Biol. 2009
10373014 RARbeta mediates the response of Hoxd4 and Hoxb4 to exogenous retinoic acid

Folberg, A, Nagy Kovács, E, Luo, J, Gigučre, V, Featherstone, MS

Dev. Dyn. 1999
19472184 p38 mitogen-activated protein kinase-dependent regulation of SRC-3 and involvement in retinoic acid receptor alpha signaling in embryonic cortical neurons

Chai, Z, Yang, L, Yu, B, He, Q, Li, WI, Zhou, R, Zhang, T, Zheng, X, Xie, J

IUBMB Life 2009
8710855 Sequence and expression of the murine Hoxd-3 homeobox gene

Tan, DP, Shao, X, Pu, L, Guo, V, Nirenberg, M

Proc. Natl. Acad. Sci. U.S.A. 1996
22872084 Anterior-posterior differences in HoxD chromatin topology in limb development

Williamson, I, Eskeland, R, Lettice, LA, Hill, AE, Boyle, S, Grimes, GR, Hill, RE, Bickmore, WA

Development 2012
23955559 Saltatory remodeling of Hox chromatin in response to rostrocaudal patterning signals

Mazzoni, EO, Mahony, S, Peljto, M, Patel, T, Thornton, SR, McCuine, S, Reeder, C, Boyer, LA, Young, RA, Gifford, DK, Wichterle, H

Nat. Neurosci. 2013
Orthologous Events